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Journal of Applied Sciences
  Year: 2020 | Volume: 20 | Issue: 1 | Page No.: 35-43
DOI: 10.3923/jas.2020.35.43
 
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Optimization of Physical Conditions of Actinokinase Synthesis from Local Isolate Using Response Surface Methodology (RSM)
Mawada Elzein Alawad Alkhider, Elrashied Elimam Elkhidir and Hanan Moawia Ibrahim

Abstract:
Background and Objective: Actinokinase is a new fibrinolytic enzymes that dissolve fibrin clots. These fibrinolytic agents have potential use to treat cardiovascular diseases, such as heart attack and stroke. This study aimed to optimize physical conditions of actinokinase extracted from local isolate in batch fermentation. Materials and Methods: Isolation and identification of the isolates were carried out using conventional technique. Enzyme was extracted and hemolytic activity of the crude enzyme and time for complete clot lysis was tested by blood agar media and test tubes containing clotted blood. Physical conditions as pH and temperature were optimized using statistical experimental design, response surface methodology (RSM). Results: About 32% of the total samples were found to be a promising enzyme producer according to hemolytic activity and large inhibition zone. Characterization of the isolates were considered as Streptomyces spp. according to microscopic and biochemical analysis. Maximum Streptomyces growth and actinokinase activity reached at 18 h of incubation. The time for complete clot lysis revealed that 20 min is the time for complete lysis of blood clot. Statistical analysis is significant with model F-value of 93.36 and R2 value of 98.53%. Optimum growth conditions of actinokinase production was at high pH of 10.6 and temperature of 36.89°C that achieve the maximum actinokinase activity to be of 47.37%. Conclusion: The optimum pH of 10.6 and optimum temperature of 36.89°C enhance three-fold actinokinase production.
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How to cite this article:

Mawada Elzein Alawad Alkhider, Elrashied Elimam Elkhidir and Hanan Moawia Ibrahim, 2020. Optimization of Physical Conditions of Actinokinase Synthesis from Local Isolate Using Response Surface Methodology (RSM). Journal of Applied Sciences, 20: 35-43.

DOI: 10.3923/jas.2020.35.43

URL: https://scialert.net/abstract/?doi=jas.2020.35.43

 
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