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Journal of Applied Sciences
  Year: 2019 | Volume: 19 | Issue: 2 | Page No.: 62-67
DOI: 10.3923/jas.2019.62.67
 
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An in vivo and in vitro Evaluation of Anti-inflammatory Action of Seeds of Vigna unguiculata Available in Bangladeshi Region

Md. Saddam Hussain , A.H.M. Mazbah Uddin, Maria Azmerin, Mohammad Tohidul Amin, Murad Hasan, Tutun Das Aka and Tanoy Mazumder

Abstract:
Background and Objective: The V. unguiculata always considered as a potential source of new drug development. Seeds of V. unguiculata have found almost all over the world but their pharmacological and nutritional value varies with geographical variability. The aim of the present study was to determine the membrane stabilizing activity of methanolic extract of V. unguiculata Linn (seed) in both in vitro and in vivo study model, while Swiss albino mice were employed as experimental animal. Materials and Methods: The V. unguiculata seeds were initially collected, air-dried by using mechanically graded aluminum foil, finally ground powder was extracted with methanol. During in vitro evaluation, five different concentrations of methanolic extract of V. unguiculata were subjected for determination of anti-inflammatory activity. In vivo evaluation of anti-inflammatory effect was tested by carrageenan induced paw edema method on Swiss albino mice model. Results: During examination of in vitro assay for membrane stabilizing activity, five different concentration of crude methanol extracts were capable of inhibiting hemolysis of erythrocyte membrane dose dependently in both hypotonic solution and heat-induced conditions, whereas in vivo anti-inflammatory test produced significant (p<0.05) inhibition of edema diameter at both doses (200 and 400 mg kg1) level, which indicates the anti-inflammatory property of the samples. Conclusion: From the above results, it is clear that V. unguiculata Linn. seeds methanolic extract showed significant anti-inflammatory potentials in both in vitro and in vivo study model. So, it will be very much possible source for an isolating lead compound for curing the inflammatory disorder.
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How to cite this article:

Md. Saddam Hussain, A.H.M. Mazbah Uddin, Maria Azmerin, Mohammad Tohidul Amin, Murad Hasan, Tutun Das Aka and Tanoy Mazumder, 2019. An in vivo and in vitro Evaluation of Anti-inflammatory Action of Seeds of Vigna unguiculata Available in Bangladeshi Region. Journal of Applied Sciences, 19: 62-67.

DOI: 10.3923/jas.2019.62.67

URL: https://scialert.net/abstract/?doi=jas.2019.62.67

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