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Journal of Applied Sciences
  Year: 2019 | Volume: 19 | Issue: 5 | Page No.: 434-440
DOI: 10.3923/jas.2019.434.440
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Non-autoclaved Sterilization Procedures of Sugarcane Tissue in vitro Culture

Suaib Suaib and Nur Fajriani Suaib

Background and Objective: In traditional plant tissue culture, sterilization medium and laboratory tools were used the autoclave procedures but this way was time consuming if compared with NaOCl system. The objectives of this observation were to shorten the sterilization procedures and to study the effect of NaOCl on in vitro callus induction (CI) and plantlet regeneration (PR) of Sugarcane plants. Materials and Methods: The laboratory tools (LT), immature leaf segments of sugarcane as explant (EP) and Murashige and Skoog basal medium (BM) were sterilized by 5.25% active chlorine (NaOCl). The different concentrations of NaOCl treatments tested were ranging from 100-500 mL L1 solute for LT and EP sterilization and 50-200 μL L1 for BM sterilization. All treatments were arranged in completely randomized design (CRD) and the percentage of LT and BM contamination, CI and PR were the observed variables. The simple mean comparison analysis was applied and to judge the best treatment of BM and EP contamination, CI and PR. Results: The best concentration of active chlorine were 300 mL L1 for LT and EP of contamination free and 100, 150 and 200 μL L1 for BM contamination-free, sugarcane CI and PR. Conclusion: Conditional protocol for non-autoclaved in vitro media, explants and laboratory tools in life span of third and callus induction and plantlets regeneration of sugarcane had successfully been achieved under active chlorine sterilization.
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How to cite this article:

Suaib Suaib and Nur Fajriani Suaib, 2019. Non-autoclaved Sterilization Procedures of Sugarcane Tissue in vitro Culture. Journal of Applied Sciences, 19: 434-440.

DOI: 10.3923/jas.2019.434.440






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