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Journal of Applied Sciences
  Year: 2019 | Volume: 19 | Issue: 5 | Page No.: 406-412
DOI: 10.3923/jas.2019.406.412
 
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A Novel Mutation in the Conserved Region of 16S rRNA Genes of Escherichia coli Clinical Isolates

Ahmed S. Kabbashi, Arwa M. Hassan, Mohammed I. Garbi , Hisham N. Altayb, Salah Eldin G Elzaki, Ashraf Siddig Yousif and Ehssan H. Moglad

Abstract:
Background and Objective: New pathogens in clinical samples that are suspected to carry bacterial infection can be effectively characterized by sequence analysis of the rrs gene (16S rRNA). This study aimed to identify and characterize Escherichia coli isolated from clinical samples (wound, urine and stool) by sequencing analysis of 16S rRNA. Materials and Methods: Escherichia coli isolated from clinical samples identified using enrichment selective media and biochemical tests. The DNA was isolated from E. coli by Chelex® method and subsequently, specific primers were used to amplify 16S rRNA genes through a conventional PCR technique. The amplified PCR product was sequenced by Macrogen Campany, Korea. The chromatogram sequences visually analyzed using Finch TV program version 1.4. The similarity and identity of the nucleotide sequence from the isolated strains compared with sequences published in the NCBI database applying the local alignment search tool BLASTn. A Phylogenetic tree generated via the Phylogeny.fr software. Results: Sequencing revealed that isolates 76 and 77 contain a novel inserted G at position 884 of reference from France (FJ544921), China (KU156692), Portugal (JQ781608), Argentina (FJ997269), Korea (FJ4638197), China (FJ803886), USA (KF574802), Korea (FJ405334), Pakistan (KR822241) and Belgium (KJ016265). Conclusion: This is one of the very few documents that shows the sequencing data of E. coli isolated from Sudanese patients and revealed a novel insertion mutation in the conserved region of 16S rRNA genes. Also, this study raises the important issue of whether conserved regions are totally conserved or not, which might have implications for the use of 16S rRNA as a biomarker, therefore, more studies are needed to confirm this result.
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How to cite this article:

Ahmed S. Kabbashi, Arwa M. Hassan, Mohammed I. Garbi, Hisham N. Altayb, Salah Eldin G Elzaki, Ashraf Siddig Yousif and Ehssan H. Moglad, 2019. A Novel Mutation in the Conserved Region of 16S rRNA Genes of Escherichia coli Clinical Isolates. Journal of Applied Sciences, 19: 406-412.

DOI: 10.3923/jas.2019.406.412

URL: https://scialert.net/abstract/?doi=jas.2019.406.412

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