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Journal of Applied Sciences
  Year: 2019 | Volume: 19 | Issue: 3 | Page No.: 199-209
DOI: 10.3923/jas.2019.199.209
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Assessment of Genetic Variability and Identification of Fertility Restoration Genes Rf3, Rf4 of WA-CMS in RILs Population of Rice

Galal Bakr Anis

Background and Objective: Development of multipurpose high-yielding rice lines is a good source of genetic variability, gene discovery and be used in two ways inbred and hybrid rice breeding. This investigation was conducted at the Experimental Farm of Sakha Agricultural Research Station, Sakha, Kafr El-Sheikh, Egypt during three seasons to study the genetic variability and identify fertility restoration genes in rice. Materials and Methods: Eighty-eight recombinant inbred lines (RILs) derived from across between PR6 (restorer) and G46B (maintainer) were evaluated for agronomic and yield-associated traits. Out of them, 20 lines as the best were selected based on their phenotypic and yield acceptability to confirm the evaluation for yield traits. Moreover, evaluation was extended to identify the fertility restoration genes Rf3 and Rf4 where located on chromosome 1 and 10, respectively. Results: Genetic variability determined in the studied traits revealed that the PCV was higher than GCV for all traits indicating that the apparent variation is not only genetic but also influenced by the growing environment in the expression of these traits. The highest broad-sense heritability values were found for growth duration, grain yield/plant and plant height. The strongest positive correlation coefficients were recorded between tillers/plant and panicles/plant and between grain yield/plant and tillers/plant, panicles/plant, spikelet fertility (%) indicating the importance of these traits as a selection criterion for yield improvement program in rice. The cluster analysis divided the 20 selected RILs into five main groups based on the mean values of the studied traits. Ten earlier reported SSR markers linked to Rf3 and Rf4 have been utilized to screen the 20 selected lines. The SSR markers RM490 and RM1108 linked to Rf3 and Rf4, respectively, identified that eight lines carry both Rf3 and Rf4 genes. Conclusion: It could be recommended that these lines can be utilized in hybrid rice breeding program as restorer lines of wild-abortive cytoplasmic male sterile lines (WA-CMS).
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How to cite this article:

Galal Bakr Anis , 2019. Assessment of Genetic Variability and Identification of Fertility Restoration Genes Rf3, Rf4 of WA-CMS in RILs Population of Rice. Journal of Applied Sciences, 19: 199-209.

DOI: 10.3923/jas.2019.199.209






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