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Journal of Applied Sciences
  Year: 2019 | Volume: 19 | Issue: 2 | Page No.: 148-155
DOI: 10.3923/jas.2019.148.155
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Alteration in Morphological and Toxicological Properties of Copper Oxide Nano-particles: The pH Effect

Zorawar Singh

Background and Objective: Copper oxide (CuO) nano-particles are being used in different fields of research. The pH of the precursor solution during nano-particle synthesis may alter their morphological as well as toxicological parameters. Materials and Methods: To evaluate the effect of precursor pH, CuO nano-particles synthesized at pH 7 and 10 of the precursor solution were procured. Both samples were characterized using scanning and transmission electron microscopy, as well as X-ray diffraction (XRD) analysis for their particle sizes. The TEM and XRD analyses revealed a smaller crystallite size of CuO nano-particles synthesized at pH 10 as compared to particles synthesized at pH 7. Different concentrations (0.1, 0.01 and 0.001 g/100 mL) were prepared and applied for exposure treatments using Allium cepa. Results: The CuO nano-particles showed a dose dependent toxicity in Allium cepa root analysis test. Significant reduction in the root lengths was observed in the treated onion bulbs as compared to the controls (1.32±0.33 vs. 4.52±0.09 cm, p<0.05). The CuO nano-particles synthesized at pH 10 were found to be more toxic as revealed by shorter root lengths as compared to pH 7 treated samples (1.32±0.33 vs. 2.94±0.67 cm, p<0.05). Conclusion: As nano-particles synthesized at higher precursor solution pH are associated with more toxicity, pH seems to play an important role in determining the particle size as well as toxicity of CuO nano-particles. Further studies are recommended to check the effect of pH on nano-particle morphology and toxicity.
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How to cite this article:

Zorawar Singh , 2019. Alteration in Morphological and Toxicological Properties of Copper Oxide Nano-particles: The pH Effect. Journal of Applied Sciences, 19: 148-155.

DOI: 10.3923/jas.2019.148.155






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