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Journal of Applied Sciences
  Year: 2018 | Volume: 18 | Issue: 1 | Page No.: 48-55
DOI: 10.3923/jas.2018.48.55
 
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Molecular Detection of Multidrug Resistance Pathogenic Bacteria from Protective Materials Used By Healthcare Workers (HCW); Bangladesh Scenario

Afroza Sultana, Otun Saha , Ashfaqur Rahman Siddiqui, Apurbha Saha, Md. Saddam Hussain and Tarequl Islam

Abstract:
Background and Objective: The increasing trend of Hospital acquired infections (HAIs), especially the ones caused by the multidrug resistant organisms has become a major public health concern. So that the aim of this study was to detect the dissemination of multidrug-resistant pathogenic bacteria on hands, gloves and masks of healthcare workers in some hospitals and pathology laboratories located in Noakhali and Dhaka, Bangladesh. Materials and Methods: A total of 106 fully fresh samples were collected. The samples were then subjected to various phenotypic cultural, biochemicals, antibiotic sensitivity along with molecular Polymerase Chain Reaction (PCR) analysis according to the standard procedures. Results: Approximately, 138 (57.5%) representative bacterial isolates were recovered among which the most frequently identified bacterium was E. coli 72 (52.17%) followed by Staphylococcus aureus 42 (30.43%), Salmonella typhi 14 (10.14%) and Pseudomonas aeruginosa 10 (7.25%). Among them total 33.33, 100 and 40% of Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa were pathogenic scanned through hlg, invA and OprI genes presence respectively. In contrast E. coli was tested through Congo red binding test where 36.11% were found pathogenic. The general frequency of Multiple Drug Resistance (MDR) pathogens were 79.31, 51.72, 68.97, 24.13, 6.89, 6.89, 17.24, 82.87, 24.13, 65.52 and 100% against ampicillin, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, methicillin, streptomycin, tetracycline, nalidixic acid, doxycycline and imipenem consecutively. Conclusion: Healthcare personnel convey multiple drug resistance pathogenic bacteria in their protective materials which are potential source of nosocomial infections. Appropriate infection prevention measures, such as good hygiene practices and training for the healthcare workers should be taken to minimize the risks that are associated with the high rate of cross-contamination.
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How to cite this article:

Afroza Sultana, Otun Saha, Ashfaqur Rahman Siddiqui, Apurbha Saha, Md. Saddam Hussain and Tarequl Islam, 2018. Molecular Detection of Multidrug Resistance Pathogenic Bacteria from Protective Materials Used By Healthcare Workers (HCW); Bangladesh Scenario. Journal of Applied Sciences, 18: 48-55.

DOI: 10.3923/jas.2018.48.55

URL: https://scialert.net/abstract/?doi=jas.2018.48.55

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