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Journal of Applied Sciences
  Year: 2017 | Volume: 17 | Issue: 9 | Page No.: 475-481
DOI: 10.3923/jas.2017.475.481
 
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Isolation and Characterization of Pathogenic Bacteria from Kundu River Water of Nandyal, Kurnool, Andhra Pradesh, India

Thamidela Sirisha, Pinjari Aleem Basha and Bommana Kavitha

Abstract:
Background and Objective: Kundu river water was used by local population for various domestic purposes previously. Now the water is getting contaminated with municipal sewage and industrial waste. Frequently, during rainy season or floods, Kundu river gets overwhelming water which cause severe damage to the local population through various infections. The main objective of the present study is to identify the pathogenic strains present in the kundu river water and how it will affect the human population using this water for various domestic purposes. Materials and Methods: The present study was performed by collecting the water samples from the Kundu river. The contaminating bacteria were isolated by following microbiological methods. The morphological and biochemical analysis was done by following standard procedures. Morphologically it has been observed in microscope and Gram staining was done to differentiate between Gram positive and negative. Biochemical analysis was done to identify the genus of the bacteria. Also done the antibiotic susceptibility test to know how harmful they can be if they infect the surrounding population. Results: Based on the morphological and biochemical analysis, it was found that the Kundu river water is highly contaminated with pathogenic bacteria. Most of the bacteria that were isolated and identified in this study belong to Escherichia coli, Klebsiealla pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Shigella dysenteriae. Many of these bacteria were showing resistance towards the antibiotics in the antibiotic susceptibility test. Conclusion: In the present study, it was identified that Kundu river water is contaminated with bacteria which may affect the population if they use this water for domestic purposes without proper treatment. Therefore, this type of research can be useful in bringing the awareness both in the Government and public regarding the safety precautions and measures to be taken to prevent contamination of water resources.
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How to cite this article:

Thamidela Sirisha, Pinjari Aleem Basha and Bommana Kavitha, 2017. Isolation and Characterization of Pathogenic Bacteria from Kundu River Water of Nandyal, Kurnool, Andhra Pradesh, India. Journal of Applied Sciences, 17: 475-481.

DOI: 10.3923/jas.2017.475.481

URL: https://scialert.net/abstract/?doi=jas.2017.475.481

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