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Journal of Applied Sciences
  Year: 2016 | Volume: 16 | Issue: 9 | Page No.: 429-437
DOI: 10.3923/jas.2016.429.437
 
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Rapid Screening of Various Factors Influencing Polysaccharides Production by Microchlorophyte Alga, Chlorella vulgaris, by Application of Plackett-Burman Design: Characterization of Polysaccharides Using High Performance Liquid Chromatography
Noura El-Ahmady El-Naggar, Mervat H. Hussein, Sami A. Shaaban-Dessuuki and Shimaa R. Dalal

Abstract:
Background: Due to high nutritional value, natural pigments and antioxidant activity, Chlorella vulgaris is used as a health food, feed supplement, as well as in the pharmaceutical and cosmetics industry. It contains polysaccharides, proteins, lipids, antioxidants, vitamins, carotenoids, immunostimulator compounds and minerals. A statistical approach has been employed for which a Plackett-Burman design is used for identifying significant variables influencing polysaccharides production by Chlorella vulgaris. Materials and Methods: Ten different independent variables including mannitol, sodium acetate, potassium citrate, NaNO3, K2HPO4, MgSO4, CaCl2.2H2O, Na2CO3, inoculum size and inoculum age were screened by Plackett-Burman experiment and High Performance Liquid Chromatography (HPLC) was used to characterize the compounds in Chlorella vulgaris polysaccharides. Results: Among the variables screened, mannitol, sodium acetate and inoculum age had significant effects on polysaccharides production. The HPLC chromatograms indicated that Chlorella vulgaris extract contain fructose, glucose, maltose and lactose with concentration of 13.259, 283.027, 26.387 and 25.489 mg g–1 dry weight, respectively. Conclusion: The results indicate that this alga contains high amount of glucose which can use in different applications.
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How to cite this article:

Noura El-Ahmady El-Naggar, Mervat H. Hussein, Sami A. Shaaban-Dessuuki and Shimaa R. Dalal, 2016. Rapid Screening of Various Factors Influencing Polysaccharides Production by Microchlorophyte Alga, Chlorella vulgaris, by Application of Plackett-Burman Design: Characterization of Polysaccharides Using High Performance Liquid Chromatography. Journal of Applied Sciences, 16: 429-437.

DOI: 10.3923/jas.2016.429.437

URL: https://scialert.net/abstract/?doi=jas.2016.429.437

 
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