Antioxidant Potential and Hepatoprotective Activity of Origanum majorana Leaves Extract against Oxidative Damage and Hepatotoxicity Induced by Pirimiphos-Methyl in Male Mice
Abstract:
The present experiment was designed to evaluate the oxidative
damage and hepatotoxicity resulting from Pirimiphos-Methyl (PM) exposure as
well as the hepatoprotective potential of Origanum majorana L. leaves
extract in male mice. The results revealed that Majorana leaves extract exhibited
antioxidant capacity manifested by inhibitory effects on DPPH, ABTS, hydroxyl
radical and reducing power in vitro. Male mice were divided into six
groups of six mice each: Control group (I) extract groups (II and III) received
an extract at doses of 150 and 300 mg kg-1 b.wt.; PM group (IV) received
PM (12.0 mg kg-1 b.wt., 1/10 LD50) in corn oil; groups
(V and VI) received PM along with the two doses of extracts. All the applications
were administered via oral route for 28 consecutive days. Exposure of mice to
PM caused significant changes in body and relative liver weights as well as
significant increases in the activity of serum enzymes alanine aminotransferases
(ALT), aspartate aminotransferases (AST), alkaline phosphatase (ALP) and lactate
dehydrogenase (LDH). Also, PM induced significant decreases in the activities
of hepatic superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase
(GPx) and in the content of glutathione reduced (GSH), however, induced a significant
increase in the level of hepatic lipid peroxidation (LPO) accompanied by histopathological
alterations in liver of male mice. Co-administration of O. majorana extract
to PM ameliorated the above-mentioned parameters. The ultimate effect was achieved
by the highest dose of the extract. It could be concluded that PM induced oxidative
damage and liver injury in male mice. However, co-administration of O. majorana
leaves extract attenuated the harmful effects of PM which may be attributed
to its antioxidant potential. Results indicated that O. majorana leaves
could be used for therapeutic option against hepatic injuries resulting from
pesticide intoxication.
Abdel-Tawab H. Mossa, Tarek M. Heikal, Samia M.M. Mohafrash and Amel A. Refaie, 2015. Antioxidant Potential and Hepatoprotective Activity of Origanum majorana Leaves Extract against Oxidative Damage and Hepatotoxicity Induced by Pirimiphos-Methyl in Male Mice. Journal of Applied Sciences, 15: 69-79.
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