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Journal of Applied Sciences

Year: 2014 | Volume: 14 | Issue: 20 | Page No.: 2615-2621
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Authors

Sophi Damayant

Stefanus Andry

Khairurrijal

Rahmana Emran Kartasasmita

Keywords

Research Article

Isolation of β-carotene from Palm (Elaeis guineensis Jacq.) Oil Using Transesterification-adsorption-desorption Method and its Characterization

Sophi Damayant, Stefanus Andry, Khairurrijal and Rahmana Emran Kartasasmita
A combination of adsorption and desorption method of a transesterification product in the process of isolation of β-carotene has been successfully carried out. Palm oil was transesterified using sodium hydroxide and methanol. The product was then adsorbed using kaolin and celite as comparison but kaolin was selected as a better adsorbent. The product was then desorbed using Soxhlet extractor with ether or maceration with n-hexane. Desorption results was purified by column chromatography and its purity was tested by HPLC. β-carotene obtained was further characterized by UV-Vis spectrophotometry, IR spectroscopy and NMR spectroscopy. The weight of filtrate and concentration of carotenoid in the sample obtained using Soxhlet’s method were 39.37 g and 184.99 ppm, whereas maceration method yielded 39.54 g and 160.66 ppm, respectively. The weight of purified β-carotene from soxhletation and maceration obtained by column chromatography were 17.43 and 16.80 mg, respectively. Isolation of β-carotene using maceration as desorption method produced β-carotene weight of 86.22 mg with 98.31% purity as tested with HPLC, whereas the isolation with soxhletation as desorption method resulted in 111.11 mg β-carotene with 97.12% purity. The results of characterization using UV-Vis spectrophotometry showed β-carotene’s absorption maxima at 450 and 477 nm. Following characterization using IR spectroscopy, absorption frequencies were shown at 2923.56 and 2854.13 cm-1 (aliphatic C-H), 1650.77 cm-1 (aliphatic C = C). 1H NMR spectrum showed a multiplicity doublet on chemical shifts at 6.26 and 6.63 ppm as the specific peak of β-carotene. In conclusion, β-carotene from palm (Elaeis guineensis Jacq.) oil using transesterification-adsorption-desorption method has been succesfully isolated and characterized.
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