Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Journal of Applied Sciences
  Year: 2014 | Volume: 14 | Issue: 18 | Page No.: 2189-2193
DOI: 10.3923/jas.2014.2189.2193
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Micropropagation of Dwarf Tree Peony from Lateral Buds

Wenqing Jia and Huichao Liu

Abstract:
Tree peony is known as china’s national flower which is very beautiful, graceful and looked as the best of all the flowers. Tree peony Var. spontanea Rehd was micropropagated by using lateral buds as explants, the effect of pruned and cut method on developing adventitious shoots was investigated, a regeneration system which is the first report was developed for dwarf peony from a lateral bud. A good disinfection protocol for lateral buds was as follows: Disinfecting with 75% alcohol for 30 sec and then soaking with 0.1% HgCl2 for 7 min adventitious shoots regenerated from lateral buds with different pruned and cut treatments and the control, the highest mean number of shoots (5.0) was obtained on pruned and cut wound. A high-frequency multiplication rate was achieved on woody plant medium supplemented with BA 3.0 mg L-1 and IAA 0.2 mg L-1 multiple shoots were formed within 5 weeks; adventious shoots were efficiently rooted (75.62%) on half-strength woody plant medium with NAA 1.0 and IBA 4.0 mg L-1 and rooted plantlets survived after acclimatization to the greenhouse.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Wenqing Jia and Huichao Liu, 2014. Micropropagation of Dwarf Tree Peony from Lateral Buds. Journal of Applied Sciences, 14: 2189-2193.

DOI: 10.3923/jas.2014.2189.2193

URL: https://scialert.net/abstract/?doi=jas.2014.2189.2193

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom