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Journal of Applied Sciences

Year: 2010 | Volume: 10 | Issue: 23 | Page No.: 3007-3013
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Authors

S. Nor Afifah

I. Darah

S. Shaida Fariza

M.K. Mohd Jain Nordin

Z. Nurul Aili

Keywords

The antimicrobial activities of methanol, diethyl ether, ethyl acetate, butanol, hexane and chloroform extracts of Halimeda discoidea were studied. These extracts were screened for the presence of antimicrobial activities against a broad spectrum of Gram-negative, Gram-positive bacteria, yeast and fungi. Hexane extract possessed the highest activities, followed by ethyl acetate and chloroform extracts, respectively. The hexane extract was active against eight bacterial and two yeast strains while ethyl acetate extract was active against four bacterial strains and chloroform extract was active against one yeast strain. Only the tested microorganisms that were susceptible to the extracts were further tested for the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentrations (MFC) test by the tube dilution method. Minimum Inhibitory Concentrations for the tested microorganisms were between 0.25-1.00 mg mL-1 while the Minimum Bactericidal and Minimum Fungicidal Concentrations were 1.00 mg mL-1 to 2.00 mg mL-1. No fungal isolates tested showed any susceptibility against any of the extracts. The activities were compared to known commercialized antibiotics, chloramphenicol for the bacteria and ketoconazole for the yeasts and fungi.
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