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Journal of Applied Sciences
  Year: 2010 | Volume: 10 | Issue: 21 | Page No.: 2701-2704
DOI: 10.3923/jas.2010.2701.2704
 
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Immobilization of Lipase from Candida rugosa on Chitosan Beads for Transesterification Reaction
M. Nasratun, A.S. Hasrul, A. Sureena, M.A. Nurul Aini, A.R. Ruwaida, M.S. Shalyda, A. Ideris, A.S. Rozaimi, J.H. Sharifuddin and N.I.A. Ahamad Nordin

Abstract:
This study is aiming to evaluate the performance of immobilized lipase on a chitosan support in transesterification reactions. Immobilized lipase improved the enzyme stability, allowing reusability, continuous operation and the possibility of better control of reactions. Chitosan offers several advantages as immobilization carrier such as versatility of available physical forms (flakes, porous beads, gel, fiber and membrane), low biodegradability and easy handling. In this study, porous bead of chitosan was used for immobilization of lipase from Candida rugosa. Lipase was immobilized by physical adsorption on chitosan beads. The ability of the immobilized lipase to catalyze transesterification of cooking oil was investigated. The important parameters like reaction time and oil to methanol molar ratios were studied to identify the performance comparison between free lipase and immobilized lipase on transesterification reaction. From the study it was found that maximum conversion of ester using immobilized lipase and free lipase were 73 and 77%, respectively. These results are obtained at optimum conditions of 1:4 molar ratios and reaction time of 48 h. As a conclusion, the chitosan beads were appear as a suitable support for immobilized lipase on transesterification reaction even though the ester conversion was lower than free lipase.
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How to cite this article:

M. Nasratun, A.S. Hasrul, A. Sureena, M.A. Nurul Aini, A.R. Ruwaida, M.S. Shalyda, A. Ideris, A.S. Rozaimi, J.H. Sharifuddin and N.I.A. Ahamad Nordin, 2010. Immobilization of Lipase from Candida rugosa on Chitosan Beads for Transesterification Reaction. Journal of Applied Sciences, 10: 2701-2704.

DOI: 10.3923/jas.2010.2701.2704

URL: https://scialert.net/abstract/?doi=jas.2010.2701.2704

 
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