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Journal of Applied Sciences
  Year: 2010 | Volume: 10 | Issue: 14 | Page No.: 1471-1475
DOI: 10.3923/jas.2010.1471.1475
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Effect of Sodium Gluconate on pH Value, Ammonia and Short Chain Fatty Acids Concentration in Batch Culture of Porcine Cecal Digesta

Theerawit Poeikhampha and Chaiyapoom Bunchasak

This study was conducted to evaluate the effect of SG supplementation in batch culture of pig cecal digesta (in vitro) on pH value, ammonia and Short Chain Fatty Acids (SCFAs) concentration. The dried ileal and cecal digesta of commercial crossbred female pigs were used as the substrate of fermentation and inoculums, respectively. The trial was divided into 5 groups according to the SG supplementations at 0, 1,250, 2,500, 5,000 or 7,500 ppm of dried ileal digesta (each group contained 6 replications). The experimental design was completely randomized design. The SG was added into each tube containing 8 mg of ileal digesta and 20 mL of inoculums. The tubes were immediately filled with CO2 and incubated at 39°C for 24 h. During the fermentation period, the samples of incubated fluid were collected at 4, 8 and 24 h for determination of pH and ammonia concentration, while SCFAs were determined at 24 h. At 8 and 24 h of fermentation period, SG supplementation (7,500 ppm) reduced pH value (p<0.01) and ammonia concentrations (p<0.05) in batch culture of porcine cecal digesta. At the end of fermentation period (24 h), supplementing SG evidently increased acetic acid, propionic acid and total SCFAs (p<0.01), although significant increase in butyric acids was not observed. It may imply that SG could be the useful prebiotic to depress pH and ammonia in cecal digesta of pigs due to high production of SCFAs.
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How to cite this article:

Theerawit Poeikhampha and Chaiyapoom Bunchasak, 2010. Effect of Sodium Gluconate on pH Value, Ammonia and Short Chain Fatty Acids Concentration in Batch Culture of Porcine Cecal Digesta. Journal of Applied Sciences, 10: 1471-1475.

DOI: 10.3923/jas.2010.1471.1475






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