Research Article

Optimization of Protein Extraction from Fish Waste using Response Surface Methodology

The aim of present study was to optimize protein extraction from Fish Waste (FW), sardine (Sardina pilchardus) using enzymatic method. In this study, enzymatic method involved two combinations of enzymes which were alcalase and protamex. Response Surface Methodology (RSM) was used to study the effect of independent variables, namely temperature (35-55°C), rotation speed (100-300 rpm), time (60-1440 min) and enzyme:substrate ratio (0.5-1.5) on protein extraction from FW. From RSM-generated model, the optimum conditions for extraction of protein from FW were identified to be at temperature 35°C in 1429 min reaction time, with rotation speed of 171 rpm and enzyme:substrate ratio of 1.50. At the optimum conditions, predicted protein yield in the extraction process was 80.75 mg mL^-1.

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H. Nurdiyana and M.K. Siti Mazlina, 2009. Optimization of Protein Extraction from Fish Waste using Response Surface Methodology. Journal of Applied Sciences, 9: 3121-3125.

DOI: 10.3923/jas.2009.3121.3125

URL: https://scialert.net/abstract/?doi=jas.2009.3121.3125

Journal of Applied Sciences

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Authors

H. Nurdiyana

M.K. Siti Mazlina

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Research Article

Optimization of Protein Extraction from Fish Waste using Response Surface Methodology

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