Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Journal of Applied Sciences
  Year: 2009 | Volume: 9 | Issue: 9 | Page No.: 1798-1800
DOI: 10.3923/jas.2009.1798.1800
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Use of Plastic Primer Container Covers as Effective Plug Molds for Pulsed-Field Gel Electrophoresis

Kek Heng Chua, Kah Heng See and S.D. Puthucheary

Abstract:
Plug molds used for PFGE can be expensive to purchase and often physically and experimentally inflexible. The use of plastic primer container covers as plug molds for Pulsed-Field Gel Electrophoresis (PFGE) is reported in this study. These plastic covers are cheap, effective and easy-to-use for processing samples for electrophoresis. Besides, the broad surface and flat area in the mold promotes faster cooling and the agar sets quickly at room temperature. Plugs are also easily removed with a spatula. These containers can be easily cleaned for re-use or disposed of as waste. Twenty samples can be prepared in one container. The PFGE results obtained by using these plastic primer-container covers as plug molds are similar to the PFGE results obtained by using commercial plug molds.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Kek Heng Chua, Kah Heng See and S.D. Puthucheary, 2009. Use of Plastic Primer Container Covers as Effective Plug Molds for Pulsed-Field Gel Electrophoresis. Journal of Applied Sciences, 9: 1798-1800.

DOI: 10.3923/jas.2009.1798.1800

URL: https://scialert.net/abstract/?doi=jas.2009.1798.1800

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom