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Journal of Applied Sciences
  Year: 2008 | Volume: 8 | Issue: 15 | Page No.: 2781-2784
DOI: 10.3923/jas.2008.2781.2784
 
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Plasmid Profiling of Klebsiella sp. and its Relation with Antibiotic Resistance at two Hospitals of Urmia (Iran)
Nima Hosseini Jazani, Mir Davood Omrani, Zahra Sabahi, Masumeh Mosavi and Minoo Zartoshti

Abstract:
The aim of the present study was to compare the antibacterial susceptibility patterns with the presence of plasmids in Klebsiella isolates. Thirty nine isolates were collected from urine specimens submitted to two educational hospitals in Urmia/Iran. The susceptibility patterns were determined against antibiotics, Plasmids were extracted by alkaline lysis method, electrophoresed and investigated by a UV transilluminator. Single digestion of plasmids with EcoRI and HincII were performed and the restriction patterns were compared using a DNA ladder. The rates of resistance were determined to antibiotics as follows: gentamicin 46.1%, tobramycin 48.7%, ceftizoxime 41%, co-trimoxazole 46.1%, amikacin 33.3%, cephtazidime 51.3%, ciprofloxacin 30.8%, kanamycin 53.8%, nalidixic acid 30.8% ampicillin 79.5% and Nitrofurantoin 41%. 25.6% of isolates harbored plasmids. Restriction enzyme analysis of plasmids showed unique pattern for all of plasmid positive isolates. There was a meaningful correlation between the presence of plasmid in isolates and resistance to the tested antibiotics. The absence of plasmids from the majority of isolates showed low typeability power of this technique, so using of other molecular typing techniques in companion with plasmid profiling and restriction enzyme analysis suggested for further studies.
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How to cite this article:

Nima Hosseini Jazani, Mir Davood Omrani, Zahra Sabahi, Masumeh Mosavi and Minoo Zartoshti, 2008. Plasmid Profiling of Klebsiella sp. and its Relation with Antibiotic Resistance at two Hospitals of Urmia (Iran). Journal of Applied Sciences, 8: 2781-2784.

DOI: 10.3923/jas.2008.2781.2784

URL: https://scialert.net/abstract/?doi=jas.2008.2781.2784

 
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