Water-soluble polysaccharides were extracted sequentially from Inula
crithmoides. Three methods were applied: H2O, K2C2O4H2OandNaOH. Each fraction was purified by precipitation
in ethanol followed by ultrafiltration of recovered solids dissolved in
water through a membrane with a molecular weight cut off of 100000, are
purified by precipitation with ethanol followed by ultrafiltration to
obtain respectively carbohydrates INPU, IOPU and IBPU. Elemental analysis
demonstrated that the extracted products were polysaccharides exempt from
proteins. Obtained products were characterised by their structural (sugar
composition, degree of methylation (DM) and molar mass of acid equivalent
unit) and macromolecular characteristics (molar mass and intrinsic viscosity).
The sugar composition indicates that all these polymers are pectins made
up mainly of acid galacturonic (between 33.8 and 64.8%). Determination
of the percentage of HG and RG-I of the functions extracted shows a mixture
of these two types of pectin. The macromolecular features of these compounds
have been characterized by size-exclusion chromatography coupled with
multi-angle laser light-scattering detection (SEC/MALLS).