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Journal of Applied Sciences
  Year: 2008 | Volume: 8 | Issue: 13 | Page No.: 2442-2448
DOI: 10.3923/jas.2008.2442.2448
 
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Fraction of Soluble Polysaccharides from Inula crithmoides by Sequential Extraction

Ismahen Kacem, Hatem Majdoub and Sadok Roudesli

Abstract:

Water-soluble polysaccharides were extracted sequentially from Inula crithmoides. Three methods were applied: H2O, K2C2O4H2O and NaOH. Each fraction was purified by precipitation in ethanol followed by ultrafiltration of recovered solids dissolved in water through a membrane with a molecular weight cut off of 100000, are purified by precipitation with ethanol followed by ultrafiltration to obtain respectively carbohydrates INPU, IOPU and IBPU. Elemental analysis demonstrated that the extracted products were polysaccharides exempt from proteins. Obtained products were characterised by their structural (sugar composition, degree of methylation (DM) and molar mass of acid equivalent unit) and macromolecular characteristics (molar mass and intrinsic viscosity). The sugar composition indicates that all these polymers are pectins made up mainly of acid galacturonic (between 33.8 and 64.8%). Determination of the percentage of HG and RG-I of the functions extracted shows a mixture of these two types of pectin. The macromolecular features of these compounds have been characterized by size-exclusion chromatography coupled with multi-angle laser light-scattering detection (SEC/MALLS).

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How to cite this article:

Ismahen Kacem, Hatem Majdoub and Sadok Roudesli, 2008.

Fraction of Soluble Polysaccharides from Inula crithmoides by Sequential Extraction

. Journal of Applied Sciences, 8: 2442-2448.

DOI: 10.3923/jas.2008.2442.2448

URL: https://scialert.net/abstract/?doi=jas.2008.2442.2448

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