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Journal of Applied Sciences
  Year: 2007 | Volume: 7 | Issue: 17 | Page No.: 2531-2534
DOI: 10.3923/jas.2007.2531.2534
 
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Simultaneous Detection of Enteropathogenic E. coli and Shiga Toxin-Producing E. coli by Polymerase Chain Reaction

N. ALHaj, N.S. Mariana , A.R. Raha and Z. Ishak

Abstract:
A PCR for detection of two categories of diarrheagenic Escherichia coli was developed. This method proved to be specific and rapid in detecting virulence genes from enteropathogenic E. coli (EPEC) (eae and bfp) and Shiga toxin-producing E. coli (STEC) (stx1, stx2 and eae) from seventy isolates of various sources. Present results confirm that it is possible and feasible to perform a simultaneous amplification of the virulence genes from two categories of diarrheagenic E. coli (STEC, EPEC) and that this technique becoming a novel diagnostic tool for future water food-borne outbreaks studies.
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How to cite this article:

N. ALHaj, N.S. Mariana , A.R. Raha and Z. Ishak , 2007. Simultaneous Detection of Enteropathogenic E. coli and Shiga Toxin-Producing E. coli by Polymerase Chain Reaction. Journal of Applied Sciences, 7: 2531-2534.

DOI: 10.3923/jas.2007.2531.2534

URL: https://scialert.net/abstract/?doi=jas.2007.2531.2534

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