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Journal of Applied Sciences

Year: 2006 | Volume: 6 | Issue: 7 | Page No.: 1576-1580
DOI: 10.3923/jas.2006.1576.1580

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Authors


Saputera


Djumali Mangunwidjaja


Sapta Raharja


Leonardus B.S. Kardono


Dyah Iswantini


Keywords


  • bioactive
  • Croton tiglium
  • extract
  • GC
  • GC-MS
Research Article

Gas Chromatography and Gas Chromatography-mass Spectrometry Analysis of Indonesian Croton tiglium Seeds

Saputera, Djumali Mangunwidjaja, Sapta Raharja, Leonardus B.S. Kardono and Dyah Iswantini
The result of phytochemical analysis showed that the hexane-soluble extract of seeds of C. tiglium contained fatty acids, terpenoids and alkaloids, while the ethanol-soluble extract of the seeds contained alkaloids, flavonoids, steroids, terpenoids and saponins. Moisture and proximate analysis showed that the seeds contained fat up to 40,1%, protein 26%, carbohydrate 15.51 and other elements such as fiber, moisture and ash. Gas Chromatography (GC) analysis on hexane-soluble extract of the seeds, using available instrument and reference-standards in our laboratory, showed 17 peaks indicating that at least the fat contained 17 compounds. Part of the compounds, 8 of them were identified as fatty acids and the 9 were unknown. The highest fatty acid level was linoleic acid (43.67%), oleic acid (19.98%) and myristic acid (7.64%). The Gas Chromatography-Mass Spectrometry (GC-MS) analysis of the hexane-soluble extract showed at least 32 compounds and the ethanol-soluble extract showed at least 25 compounds.
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How to cite this article

Saputera, Djumali Mangunwidjaja, Sapta Raharja, Leonardus B.S. Kardono and Dyah Iswantini, 2006. Gas Chromatography and Gas Chromatography-mass Spectrometry Analysis of Indonesian Croton tiglium Seeds. Journal of Applied Sciences, 6: 1576-1580.

DOI: 10.3923/jas.2006.1576.1580

URL: https://scialert.net/abstract/?doi=jas.2006.1576.1580

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