Research Article

**Genetic Relatedness Among Isolates of Acanthamoeba Based on RAPD Analysis**

Siti Ruhaya Abdul Manaf, Shuhaimi Mustafa, Nakisah Mat Amin and Abdul Manaf Ali

Acanthamoeba is a pathogen frequently infecting brain, eyes, skin and lung of human and animal. Seven Acanthamoeba local isolates from various sources, two Acanthamoeba type strains and one strain of Hartmannella vermiformis were characterized for their genetic variability using randomly amplified polymorphic DNA (RAPD) technique using four different 10-mer oligonucleotides primers. Electrophoresis of the amplification products generated DNA bands ranging from approximately 0.25 to 7.50 kbp in size. A genetic relatedness among the isolates was examined using Dice similarity coefficient as the genetic distance measured between the strains of Acanthamoeba and H. vermiformis. Three distinct clusters could be separated at genetic distance of approximately 0.330.

PDF Fulltext XML References Citation

How to cite this article

Siti Ruhaya Abdul Manaf, Shuhaimi Mustafa, Nakisah Mat Amin and Abdul Manaf Ali, 2006. Genetic Relatedness Among Isolates of Acanthamoeba Based on RAPD Analysis. Journal of Applied Sciences, 6: 15-19.

DOI: 10.3923/jas.2006.15.19

URL: https://scialert.net/abstract/?doi=jas.2006.15.19

Journal of Applied Sciences

Article Trend

Total views 302

Search

Authors

Siti Ruhaya Abdul Manaf

Shuhaimi Mustafa

Nakisah Mat Amin

Abdul Manaf Ali

Keywords

Research Article

**Genetic Relatedness Among Isolates of Acanthamoeba Based on RAPD Analysis**

How to cite this article

Leave a Comment