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Journal of Agronomy
  Year: 2013 | Volume: 12 | Issue: 1 | Page No.: 11-19
DOI: 10.3923/ja.2013.11.19
 
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Assessment of Two Transgenic Tobacco Plant Varieties for the HBsAg-Specific Plantibody Production

Leonardo Gomez, Sigifredo Padilla, Alejandro Fuentes, Yoslaine Ruiz, Tatiana Gonzalez, Margarita Somoza, Lisette Lopez, Julio Sanchez, David Gavilan, Elio Espinosa, Yenisleydis Avila, Otto Mendoza, Yordanka Masforrol, Cristina Garcia, O. Maylin La and Rodolfo Valdes

Abstract:
Transgenic plants are an attractive alternative to produce antibodies for the manufacturing of biologics. The main subject of this study was to assess two non-commercial transgenic tobacco plant varieties (BHmN and Habana 92) cultivated in zeolite and confined conditions for the production of a hepatitis B virus surface antigen (HBsAg)-specific plantibody (PHB-01). The plantibody molecule biochemical characterization and the assessment of the immunopurification capacity of the antigen recognized by PHB-01-immunoadsorbents were other subjects studied in this study to decide which variety would be more suitable for PHB-01 large-scale production. As results, the BHmN variety allowed obtaining 1.18 fold more biomass of leaves and up to 2 fold in the pantibody yield with similar specificity and affinity constant by the HBsAg. The assessment of the HBsAg immunopurification capacity of the PHB-01 immunoadsorbents produced by these two non-commercial transgenic plant varieties did not showed significant differences in terms of adsorption capacity (p = 0.2135), elution capacity (p = 0.1239), recovery (p = 0.2655) and purity during 13 purification cycles. In conclusion, the BHmN variety would be the most suitable variety for the production of the PHB-01.
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How to cite this article:

Leonardo Gomez, Sigifredo Padilla, Alejandro Fuentes, Yoslaine Ruiz, Tatiana Gonzalez, Margarita Somoza, Lisette Lopez, Julio Sanchez, David Gavilan, Elio Espinosa, Yenisleydis Avila, Otto Mendoza, Yordanka Masforrol, Cristina Garcia, O. Maylin La and Rodolfo Valdes, 2013. Assessment of Two Transgenic Tobacco Plant Varieties for the HBsAg-Specific Plantibody Production. Journal of Agronomy, 12: 11-19.

DOI: 10.3923/ja.2013.11.19

URL: https://scialert.net/abstract/?doi=ja.2013.11.19

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