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International Journal of Zoological Research
  Year: 2005 | Volume: 1 | Issue: 1 | Page No.: 74-83
DOI: 10.3923/ijzr.2005.74.83
Cytochemical Properties of Earthworm Coelomocytes Enriched by Percoll
Sherifa S. Hamed, EIIen Kauschke and Edwin L. Cooper

Abstract:
Coelomocytes of E. fetida were separated by Percoll gradient and based on cytomorphology and cytochemistry, classified into four major categories: acidophils, basophils, chloragocytes cells and neutrophils. Basophils exhibited heterogeneity with respect to staining properties of granules. The enzyme acid phosphatase was present in all coelomocytes, but was especially abundant in basophils and neutrophils. Alkaline phosphatase was detected in basophils and acidophils and -esterase was found in all types except neutrophils. Acidophils and basophils possessed the corresponding granules and neutrophils contained both; acidophilic granules are often excreted. Basophils and neutrophils were more active in killing the tumor target, K562, which partially reflects their role in the earthworm`s immune system.
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How to cite this article:

Sherifa S. Hamed, EIIen Kauschke and Edwin L. Cooper , 2005. Cytochemical Properties of Earthworm Coelomocytes Enriched by Percoll. International Journal of Zoological Research, 1: 74-83.

DOI: 10.3923/ijzr.2005.74.83

URL: https://scialert.net/abstract/?doi=ijzr.2005.74.83

 
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