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International Journal of Virology
  Year: 2018 | Volume: 14 | Issue: 1 | Page No.: 1-12
DOI: 10.3923/ijv.2018.1.12
Natural Infection and Recombination Analysis of Bipartite Begomovirus and its Cognate Beta-satellite in Benincasa hispida
Savaas Umar Mohammed Riyaz , Denison Michael Immanuel Jesse, Sundarajan Deepan, Thangavelu Raja Muthuramalingam, Ganapathy Rajendiran and Krishnan Kathiravan

Abstract:
Background and Objective: Whitefly-transmitted begomoviruses are the most important limiting factor in ash gourd cultivation in India. This work enlightened about the adaptability and evolution of new begomovirus complexes by interactions between begomoviruses and satellites by mixed infection and recombination. Materials and Methods: Full length genome of bipartite Squash leaf curl China virus (SLCCNV) was amplified by RCA, subsequently its cloned in pBSKII+ vector and sequenced, with universal beta-satellite primers. Croton yellow vein mosaic beta-satellite (CroYVMB), β-satellite and recombination analysis was done through RDP4. Results: Benincasa hispida was a new host for begomovirus, the presence of two different begomovirus species was identified as SLCCNV isolate KP1 and CroYVMB in B. hispida infection was observed. Recombination analysis would serve as a possible evidence for interspecies recombination between the three begomoviral entities-ToLCNDV, SLCCNV and CroYVMB that had been suspected for SLCCNV-KP1infection in the collected B. hispida. Conclusion: Novel recombination was found in DNA-A and DNA β while DNA B was not recombinant; which helped to reduce the host range and severity of the infestation. This frequency of emerging new SLCCNV strains in ash gourd indicating that the virus species had undergone recombination.
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How to cite this article:

Savaas Umar Mohammed Riyaz, Denison Michael Immanuel Jesse, Sundarajan Deepan, Thangavelu Raja Muthuramalingam, Ganapathy Rajendiran and Krishnan Kathiravan, 2018. Natural Infection and Recombination Analysis of Bipartite Begomovirus and its Cognate Beta-satellite in Benincasa hispida. International Journal of Virology, 14: 1-12.

DOI: 10.3923/ijv.2018.1.12

URL: https://scialert.net/abstract/?doi=ijv.2018.1.12

 
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