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International Journal of Virology
  Year: 2017 | Volume: 13 | Issue: 2 | Page No.: 68-79
DOI: 10.3923/ijv.2017.68.79
Pharmacological Evaluation of the Hibiscus Herbal Extract Against Herpes Simplex Virus-type 1 as an Antiviral Drug in vitro
Zenab Aly Torky and Mohammad Mofazzal Hossain

Background and Objective: High prevalence of viral infections and constant emergence of resistant virus strains worldwide calls for an urgent need to develop therapeutic agents to improve the treatment of virus infection especially against Herpes Simplex Virus-type 1. The objective of this study was to conduct phytochemical screenings of the methanolic/aqueous Hibiscus extracts and evaluate their antiviral activity as well as their therapeutic and prophylactic activity. Materials and Methods: Identification and evaluation of phytochemical ingredients and essential components of the Hibiscus extracts through different chemical analytical methods were conducted. Antiviral activity of Hibiscus extracts was evaluated using plaque reduction assay of the viral cytopathic effect as well as cell viability and proliferation assays using MTT, to evaluate the extract feasibility as a potential therapeutic agent. Experiments like viral binding and adsorption, virucidal and time of addition assays were designed to investigate the mechanism of antiviral activity of Hibiscus. Moreover, viral kinetics and antigen expression were demonstrated by fluorescence microscopy. MS Excel 2010 was used to provide statistical analysis like average and standard deviation. Results: Phytochemical analysis succeeded in extracting major constituents of Hibiscus like polyphenols, flavonoids and anthocyanin. Hibiscus showed a potential activity of methanolic and aqueous extracts against HSV-1 as indicated by selectivity index values of 8.0 and 7.7 correspondingly. Mechanism of action, revealed that the extract not only prevented the virus particles from interacting with the Vero cells in the pre-treatment assay but also had a prophylactic effect blocking the replication of the virus causing prophylactic selectivity indices of 6.1 and 5.2 for methanolic and aqueous extracts correspondingly, confirming a maximum protection of Vero cells against HSV-1 attack. Antigen expression Results showed that the effect of Hibiscus on inhibiting the HSV-1 virus after 5 h is much better than their effect 10 h post infection. Conclusion: Results demonstrated the potent and broad spectrum antiviral activity of Hibiscus due to the multiple components contained in the extract from active ingredients which cooperate together in synergetic manner and that all Hibiscus extracts tested were effective as virucidal and prophylactic agent, which makes Hibiscus a novel treatment against HSV-1.
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How to cite this article:

Zenab Aly Torky and Mohammad Mofazzal Hossain, 2017. Pharmacological Evaluation of the Hibiscus Herbal Extract Against Herpes Simplex Virus-type 1 as an Antiviral Drug in vitro. International Journal of Virology, 13: 68-79.

DOI: 10.3923/ijv.2017.68.79


27 January, 2018
My name Cindy Lucas from US taxes for a year now i have been living with this virus called (Herpes) VIRUS and i have been taking some medications from my Doctor and there was no improvement, All thanks to God almighty for using Dr Abudu for curing my (Herpes) Virus with the help of his powerful root and herbs...Well i do not have much to say, with all my life i will for ever be grateful to him and God Almighty for using Dr, Abudu to reach me when i thought it is all over, today i am now happy after the medical doctor have confirmed my status Negative, i have never in my life believed that (Herpes) could be cure by any herbal medicine.
10 October, 2018
Meg Fullor:
When i first heard about herbal remedy i never believe on such word until i found dr ehi a great traditional doctor that was introduced to me by a friend that suffered from Hepatitis B and was cure with the help of this great herbal doctor (dr ehi) so i decided to contact him for help in getting rid of my families genital herpes virus 1/2 which i did and all i was told to provide was just some useful information and some materials use in preparation of the natural cure and that i did and now i am the happiest person on earth because i am writing this testimony of mine with joy and happiness in my heart to the whole world to know that natural remedy is real because i am a living testimony of dr ehi's traditional herbal cure and i want you all to contact dr ehi. I am sure he will help you too.
11 October, 2018
preshy oboite:
i am from UNITED STATES i was diagnosed with HIV/AIDS for over 12 years which made loose my job and my relationship with my fiance after he discovered that i was having HIV, he departed from me i tried all my best to make him stays with me, but neglected me until a friend of mine on Facebook from Singapore told me Great healer, who will restore my life back with his powerful healing spell, he sent me the email address to contact and i quickly contacted him, and he said my condition can solved, that he will treat the disease immediately only if i can accept trust him and accept his terms and condition, i Agreed because i was so much in need of help by all means, so i did all he want from me and surprisingly on Sunday last week He sent me a text, that i should hurry up to the hospital for a checkup, which i truly did, i confirm from my doctor that i am now ( HIV NEGATIVE) my eyes filled with tears and joy, crying heavily because truly the disease deprived me of many things from my life, This is a Miracle
12 October, 2018
I was diagnosed of herpes virus in 2016, I have tried all possible means to get cure but all my effort proved abortive, until a friend of mine introduced me to a herbal doctor called dr. Alli, who prepare herbal medicine to cure all kind of diseases including herpes virus (Herpes), when i contacted this herbal doctor, he sent me herpes virus herbal medicine via courier service, when i received the herbal medicine he gave me step by step instructions on how to apply it, when i applied it as instructed i was totally cured from the virus within 3 weeks of usage
25 October, 2018
Eric Scott:
I was diagnosed of herpes 3 years, and ever since then i have been taking treatment to prevent outbreaks, burning and blisters, but there was no improvement until i came across testimonies of Dr. Okasi on how he has been curing different people from different diseases all over the world, then i contacted him. After our conversation he sent me the medicine which i took according to his instructions for up to 3 weeks. After completing the medication i went back to my doctor for another test and the virus was all gone and i was completely cured, since then i have not had any signs of outbreak. I'm so filled with joy. With herbal medication Herpes Virus is 100% curable.






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