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International Journal of Virology
  Year: 2014 | Volume: 10 | Issue: 4 | Page No.: 272-279
DOI: 10.3923/ijv.2014.272.279
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Indexing of Potato leaf roll virus (PLRV) from Potato Growing Areas of Punjab, India
Balwinder Singh

Potato leaf roll virus (PLRV) is a serious disease of potato causing decline in potato production worldwide. Symptoms of PLRV like leafroll and stunting were observed during survey in potato fields of Punjab. Tubers and leaves of symptomatic potato plants were indexed for PLRV using test plants, DAS-ELISA and RT-PCR. Seedlings of Phaseolus floridana and Datura stramonium were used as test plants and they showed stunted growth and chlorotic spots after 10-12 days of inoculation feeding by aphid vector (Myzus persiace). P. floridana developed more visible symptoms of PLRV infection as compared to D. stramonium. Sample collected during survey from potato growing areas and test plants showing symptoms of virus after aphid transmission were indexed by DAS-ELISA. All test plants showing symptoms of virus and 78.57% of leaves and 74.41% of tuber samples colleted during survey were detected positive for virus in this serological method. The RT-PCR assay of ELISA positive samples yielded amplification of 336 bp corresponding to partial coat protein gene of PLRV. The present study reports the prevalence of PLRV in potato growing areas of Punjab.
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How to cite this article:

Balwinder Singh , 2014. Indexing of Potato leaf roll virus (PLRV) from Potato Growing Areas of Punjab, India. International Journal of Virology, 10: 272-279.

DOI: 10.3923/ijv.2014.272.279








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