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International Journal of Virology
  Year: 2012 | Volume: 8 | Issue: 1 | Page No.: 81-89
DOI: 10.3923/ijv.2012.81.89
Molecular Variation of Potato virus Y Isolated from Egypt
E.A. El-Absawy, Amal Mahmoud, A.A. Hemeida and M. Helmy

Abstract:
Potato Virus Y (PVY) is one of the most important viruses affecting cultivated potatoes in Egypt. Different potato plants were collected from an experimental station in Giza Governorate, Egypt and were tested using RT-PCR. PVY was amplified using primers represented portion of the Coat Protein (CP) gene and 3' Untranslated Regions (UTR). Phylogenetic tree showed two main strain groups: Group I regroups PVYN and PVYNTN stains while Group II includes PVYO, PVYW and PVYN:O strains. The Egyptian PVY isolate was clearly classified within group I and was more closely related to PVYNTN strains. Ten nucleotide substitutions resulted in 3 conserved amino acid substitutions (V1→I, G7→E, M or V and S8→G) and were able to differentiate between both groups. The partial coat protein region was more diverse than that of the 3'UTR (92.6-100% and 97.7-100% identity, respectively). The 3'UTR of the Egyptian isolate showed RNA secondary structures different from those of the 5 PVY strains.
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How to cite this article:

E.A. El-Absawy, Amal Mahmoud, A.A. Hemeida and M. Helmy , 2012. Molecular Variation of Potato virus Y Isolated from Egypt. International Journal of Virology, 8: 81-89.

DOI: 10.3923/ijv.2012.81.89

URL: https://scialert.net/abstract/?doi=ijv.2012.81.89

 
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