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International Journal of Virology
  Year: 2012 | Volume: 8 | Issue: 3 | Page No.: 271-278
DOI: 10.3923/ijv.2012.271.278
Establishment of Dot-blot Hybridization for Diagnosis of Bovine Ephemeral Fever Virus in Egypt
A.H. Zaghloul, Amal Mahmoud , H.Y. Hassan , A.A. Hemeida , M.A. Nayel and A.A. Zaghawa

Abstract:
Bovine Ephemeral Fever (BEF) is an arthropode borne rhabdo virus, which causes a disabling febrile infection of cattle and water buffalo. A sever outbreak was recorded in Egypt during 2000 and 2001. Using RT-PCR, a primer designed to amplify 438 nucleotide fragments from the glycoprotein G gene allowed specific amplification of BEFV-cell culture isolates from Egypt (Menoufia and Alexanderia Governorates) and Japan. The PCR product was sequenced and analyzed. PCR product of Alexanderia isolate was cloned and labeled with digoxigenin and used as diagnostic probe for BEF virus infection using dot-blot hybridization. Thirty six samples were collected from Menoufia Governorate (Berket El-Sabaa, Tokh Tambesha and Salamon regions) and Demiate Governorate and were tested in dot-blot hybridization. BEFV was detected in 24 out of 36 samples (66.7%) as follow: 9/9 (100%) correspond to Berket El-Sabaa and Tokh Tambesha regions and 3/9 (33.3%) correspond to Demiate and Salamon regions. In conclusion, a local Egyptian probe was designed and applied in the dot blot hybridization technique for diagnosis of bovine ephemeral fever virus.
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How to cite this article:

A.H. Zaghloul, Amal Mahmoud , H.Y. Hassan , A.A. Hemeida , M.A. Nayel and A.A. Zaghawa , 2012. Establishment of Dot-blot Hybridization for Diagnosis of Bovine Ephemeral Fever Virus in Egypt. International Journal of Virology, 8: 271-278.

DOI: 10.3923/ijv.2012.271.278

URL: https://scialert.net/abstract/?doi=ijv.2012.271.278

 
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