Sahar A. Youssef
Agricultural Research Center, Department of Virus and Phytoplasma Research, Plant Pathology Research Institute, 9 Gamaa Street, 12619, Giza, Egypt
A.A. Shalaby
Agricultural Research Center, Department of Virus and Phytoplasma Research, Plant Pathology Research Institute, 9 Gamaa Street, 12619, Giza, Egypt
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How to cite this article
Sahar A. Youssef and A.A. Shalaby, 2009. Single-Step Multiplex Reverse Transcription-Polymerase Chain Reaction (m-RT-PCR) for Simultaneous Detection of Five RNA Viruses Affecting Stone Fruit Trees. International Journal of Virology, 5: 100-108.
DOI: 10.3923/ijv.2009.100.108
URL: https://scialert.net/abstract/?doi=ijv.2009.100.108
DOI: 10.3923/ijv.2009.100.108
URL: https://scialert.net/abstract/?doi=ijv.2009.100.108
mohamad rezaee Reply
Hi my dear
I want information about plant virus diseases, their detection methods, symptoms and methods of acid nucleic extraction.
M.rezaee
plant protection office
Agriculture ministry
North Khorasan
Iran
Aboulata Elnady ABOUL-ATA
Dear Sir.
The comment you sent for answering is an ordinary subject.
Because you did not identify which virus you would like me to talk about!!. So, you can find all those information in too many websites about Plant Virus Identification and Detection. Also, you can search by the Plant Virus name as well.
Also, you can go and read basic information on Virology and Plant Viruses.
Finally, the points you asked for could build a small book. So, just try what I've directed and come back if any more problems with no hisitation.
Thank you for sending your comment.
AE Aboulata
Aboul-Ata Elnady ABOUL-ATA
Dear Sir. I have done my comment because there was no naming the virus or viruses.
mohamad rezaee Reply
Thank you for your reply. I am planing a research of viruses detection of stone and pome fruits and grape in my province in Iran.My request are included:
1- Main those plants viruses names and sequencing nucleotide for primer synthesis.
2- Methods of extraction nucleic acid and PCR programming.
3-Information about those viruses for literature review.
The best wish
Aboul-Ata Elnady ABOUL-ATA
Dear Sir.
OK, I'll send you the information you need and the sources you can go to. Just give me enough time (2 weeks).
By the way, do you need to have only information on viral infection or on viroid infection??
Have a good day
AE Aboulata