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International Journal of Virology

Year: 2007 | Volume: 3 | Issue: 1 | Page No.: 28-34
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Authors

Ashraf A. Tabll

Mohamed El-Sadany

Keywords

Research Article

Serotype Analysis of Hepatitis C Virus in Patients with Liver Cirrhosis Positive and Negative for HCV RNA Using Enzyme Immunoassay

Ashraf A. Tabll and Mohamed El-Sadany
Hepatitis C virus serotype analysis of selected specimens from patients with liver cirrhosis positive and negative for HCV RNA was determined. Twenty four serum specimens were evaluated and stored at -20°C. The specimens all tested positive for HCV antibodies on a screening enzyme immunoassay, with confirmation on a recombinant immunoblot assay (RIBA). All specimens were also tested for HCV RNA by the reverse transcription polymerase chain reaction (RT-PCR). The sera were submitted to ELISA, modified, for the identification of antibodies against HCV serotypes 1, 2, 3, 4, 5 and 6 (Murex HCV serotyping l-6 assay). Out of 24 serum samples, 3 samples were undetectable. Type 4 predominated (81%), followed by type 2 (19%), types 1, 3, 5 and 6 were did not detected in the tested samples. Mixed HCV serotype 4 and 2 were detected in 3 patients (14.2%). All positive HCV antibodies sera were also positive by RIBA test. Of 21 serum samples positive for HCV antibodies, 13 samples (13/21, 61.9%) were positive for HCV RNA. HCV serotype 4 was found positive in 10/13 (76.9%) of positive HCV RNA. In negative HCV RNA serum samples (n = 8), HCV serotype 4 were found positive in 6/8 (75%). In conclusions, HCV Serotype 4 is the predominate type in the tested patients. Serotype 1, 3, 5 and 6 were not detected in the tested samples Analysis of HCV serotyping was possible in patients who had negative for HCV RNA from their serum.
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