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International Journal of Virology
  Year: 2007 | Volume: 3 | Issue: 3 | Page No.: 100-106
DOI: 10.3923/ijv.2007.100.106
A TaqManR RT-PCR Assay for the Detection of Feline calicivirus
Yogesh Chander, Ashok K. Tiwari, Suchitra Sajja, M.A. Ramakrishnan, Kay S. Faaberg and Sagar M. Goyal

Abstract:
Feline calicivirus (FCV) is one of the most common pathogens of cats causing upper respiratory tract infections. A real time RT-PCR assay was developed for the rapid detection of FCV. A set of probe/primers was designed to amplify a region of 151 bp based on the conserved region of 7 FCV strains (FCV 255, 2280, NADC, F9, KCD, CF I and Urbana) by sequence comparison. The assay was found to be more sensitive than virus isolation and was linear over a wide range of template concentrations.
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How to cite this article:

Yogesh Chander, Ashok K. Tiwari, Suchitra Sajja, M.A. Ramakrishnan, Kay S. Faaberg and Sagar M. Goyal, 2007. A TaqManR RT-PCR Assay for the Detection of Feline calicivirus. International Journal of Virology, 3: 100-106.

DOI: 10.3923/ijv.2007.100.106

URL: https://scialert.net/abstract/?doi=ijv.2007.100.106

 
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