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International Journal of Virology
  Year: 2006 | Volume: 2 | Issue: 1 | Page No.: 59-62
DOI: 10.3923/ijv.2006.59.62
 
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Absence of DNA Human Papillomavirus Type 16 in Mexican Women with Normal Pap Smear
Maria del Refugio Gonzalez-Losa, Ana C. Hidalgo-Martinez , Luis Manzano-Cabrera , Aremi Estrada-Saldivar and Marylin Puerto-Solis

Abstract:
To determine the prevalence of human papillomavirus type 16 in women with normal pap-smear. All women self-refering for pap smear were enrolled sequentially during 6 months. During the pap smear, an extra sample of cervical cells were taken and suspended in cold PBS. HPV diagnosis was done by polymerase chain reaction using MY09/MY11 primers. Genotyping was done by specific nested PCR. Four hundred ninety five women were included in the study, 80 (16.16%) were negative to β-globin. The prevalence of HPV was 6.5%. Any sample amplified with specific PCR. The imminent use of the vaccine against HPV 16 makes indispensable epidemiological studies in focus to have data of HPV 16 prevalence and distribution in representative samples with the same methodology in order to have a solid evidence to evaluate the results of HPV vaccination.
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How to cite this article:

Maria del Refugio Gonzalez-Losa, Ana C. Hidalgo-Martinez , Luis Manzano-Cabrera , Aremi Estrada-Saldivar and Marylin Puerto-Solis , 2006. Absence of DNA Human Papillomavirus Type 16 in Mexican Women with Normal Pap Smear. International Journal of Virology, 2: 59-62.

DOI: 10.3923/ijv.2006.59.62

URL: https://scialert.net/abstract/?doi=ijv.2006.59.62

 
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