Immunization with DNA Vectors Consisting of Selected Dengue 2 Virus Genes Stimulated Antibody Responses in Mice


	International Journal of Virology
	Year: 2006 \| Volume: 2 \| Issue: 1 \| Page No.: 14-20 DOI: 10.3923/ijv.2006.14.20

Immunization with DNA Vectors Consisting of Selected Dengue 2 Virus Genes Stimulated Antibody Responses in Mice

Norazizah Shafee and Sazaly AbuBakar

Abstract: Dengue virus Type 2 C, prM, truncated E (NB-E and B-E), NS1, NS2A, NS2B/3 and NS3 genes were cloned and expressed using the pEGFP-N1 eukaryotic expression vector. Protein expression was visualized in transfected Vero cell cultures. Immunogenicity of the recombinant DNA plasmids was determined by inoculating 8 weeks old BALB/cJ mice with two doses of plasmid cocktail followed by a booster inoculation at two weeks apart. Mice were also injected with empty vector plasmids or vaccinated with virus inoculum as controls. Initial inoculation with the plasmid cocktail resulted in IgG response comparable to inoculation with virus inoculum alone which peaked after the booster dose. Rapid rise in IgG response was also observed when the mice were subsequently challenged with partially purified dengue virus Type 2, ten weeks after the first inoculation. These results suggest for the first time, that dengue virus DNA genes expressing antigens as fusion proteins with EGFP could stimulate immune responses in vivo.

[Fulltext PDF] [Fulltext HTML] [XML: Abstract + References] [References] [View Citation] [Report Citation]

RELATED ARTICLES:

•	How to Cope with Dengue in Developing Countries Like Pakistan?

•	Characterization of Dengue Type 2 NGC Virus Infection in C6/36, Vero and MRC-5 Cells

How to cite this article:

Norazizah Shafee and Sazaly AbuBakar , 2006. Immunization with DNA Vectors Consisting of Selected Dengue 2 Virus Genes Stimulated Antibody Responses in Mice. International Journal of Virology, 2: 14-20.

DOI: 10.3923/ijv.2006.14.20

URL: https://scialert.net/abstract/?doi=ijv.2006.14.20

COMMENT ON THIS PAPER

	Full Name:
	E-mail:
	Comments:

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		SCIMAGO
		SCOPUS