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International Journal of Soil Science
  Year: 2007 | Volume: 2 | Issue: 4 | Page No.: 308-313
DOI: 10.3923/ijss.2007.308.313
 
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Production of Extracellular Polysaccharide by Rhizobium Strains from Root Nodules of Leguminous Green Manure Crop, Sesbania sesban (L.) Merr.

M. Sridevi and K.V. Mallaiah

Abstract:
The ability of twenty six Rhizobium strains, isolated from root nodules of leguminous crop, Sesbania sesban (L.) Merr. were tested for their production of extracellular polysaccharides (EPS) in Yeast Extract Mannitol (YEM) medium. Among the twenty six strains, maximum amount of EPS was produced by Rhizobium SS5 strain (3900 μg mL-1). Both growth and EPS production started simultaneously, but the EPS production was maximum in the stationary phase of growth (48 h). The EPS production was maximum, when the medium was supplemented with galactose (2%), sodium nitrate (0.1%) and Ca-pantothenate (1 μg mL-1), which was accompanied by a great increase in the production compared to the control. The EPS contained galactose, glucose, xylose, rhamnose and raffinose, which were identified by paper as well as gas liquid chromatography.
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How to cite this article:

M. Sridevi and K.V. Mallaiah , 2007. Production of Extracellular Polysaccharide by Rhizobium Strains from Root Nodules of Leguminous Green Manure Crop, Sesbania sesban (L.) Merr.. International Journal of Soil Science, 2: 308-313.

DOI: 10.3923/ijss.2007.308.313

URL: https://scialert.net/abstract/?doi=ijss.2007.308.313

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