Background and Objective: Pituitary-specific Transcription Factor polymorphism gene of livestock can be used as a basis for selection programs. A study was conducted to categorize 6 Pituitary-Specific Transcription Factor (Pit-1|TasI) polymorphism genes and their associations with body weight of Super kampung chicken. Materials and Methods: Day-old 112 Super Kampung chickens were used in this study. Chicks were kept in an open house with slat floor until the age of 13 weeks. Feed and drinking water were provided ad libitum. Blood sample was collected with a syringe (3 mL) from the brachial vein and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was used for analysis. Results: The results showed that the Pit-1 genes were polymorphic in Super Kampung chickens. There were two alleles, namely C and T, each with a rate of 0.746 and 0.254, respectively and three genotypes, namely CC, CT and TT, each with a frequency of 0.598, 0.295 and 0.107, respectively. The Pit-1 gene in the Super kampung chicken population was not in equilibrium. There were correlations between the genotype of fragment genes Pit-1|TasI exon 6 and the body weight of the Super Kampung chickens at 13 weeks of age. The highest body weight was found in the CC genotype. Conclusion: The CC genotype of the Pit-1 gene can be used as a growth marker in Super Kampung chickens.
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