Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Poultry Science
  Year: 2014 | Volume: 13 | Issue: 4 | Page No.: 240-245
DOI: 10.3923/ijps.2014.240.245
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Genetic Relatedness among Helicobacter pullorum Isolates from Broiler Chickens
Soe Soe Wai, A.A. Saleha, Z. Zunita, L. Hassan, A. Jalila and A.H. Shah

Abstract:
Helicobacter pullorum, an enterohepatic helicobacter with the ability to colonize the intestine and liver of avian species, mice and humans, is increasingly recognized as a food borne zoonotic pathogen. The present study aimed to determine the genetic similarity among H. pullorum using pulsed field gel electrophoresis (PFGE) with SacII and SmaI restriction endonucleases (REs) digestion. The H. pullorum were isolated from broiler chickens in eight farms. Within-farm genetic profiling of isolates showed close relationship (>95%); however genetic diversity was observed between isolates from different farms. Digestion of genome with SacII yielded two clusters comprised 16 pulsotypes, 8-14 DNA bands with a molecular weight ranging from 40 to 400 kb whereas SmaI digested genome produced two clusters with 14 pulsotypes, 7 to 13 DNA bands with molecular weight ranging from 40 to 250 kb. The RE SacII showed a higher discriminatory power compared to SmaI. In conclusion, within-farm genetic similarity of isolates suggested the same source of clonal origin.
PDF References Citation Report Citation
 RELATED ARTICLES:
  •    Helicobacter pullorum among Poultry in Assiut-Egypt: Genetic Characterization, Virulence and MIC
How to cite this article:

Soe Soe Wai, A.A. Saleha, Z. Zunita, L. Hassan, A. Jalila and A.H. Shah, 2014. Genetic Relatedness among Helicobacter pullorum Isolates from Broiler Chickens. International Journal of Poultry Science, 13: 240-245.

DOI: 10.3923/ijps.2014.240.245

URL: https://scialert.net/abstract/?doi=ijps.2014.240.245

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 

       

       

Curve Bottom