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| Molecular Identification of Campylobacter jejuni and Campylobacter
coli Isolated from Small-Scale Poultry Slaughterhouse in Lima, Peru |
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Cesar A. Lazaro,
Carlos A. Conte-Junior,
Miguel A. Vilca,
Juan R. Lucas,
Daphne D. Ramos,
Alberto Manchego,
Kim R.L. Chiok and Robson M. Franco |
Abstract:
A total of 96 samples (3 scalding water, 3 final washing water,
30 non-eviscerated, 30 eviscerated and 30 cecal content) from three small-scale
broiler slaughterhouses were evaluated. Bacteriological test was performed with
mCCDA medium and positive samples were confirmed by PCR assays using 16S rDNA,
hipO and asp primers to Campylobacter spp., Campylobacter
jejuni and Campylobacter coli respectively. Bacteriological test
showed the presence of Campylobacter spp. in 60 samples. However, according
to the PCR assays, only 19 samples were confirmed as positive. Non-eviscerated
and eviscerated carcasses had fewer numbers of samples positive for Campylobacter
spp. (63 and 50%, respectively) than the cecal content samples (77%). No positive
were found in the scalding and the final washing water samples. No differences
(P>0.05) were observed between PCR and biochemical tests for Campylobacter
jejuni and Campylobacter coli identification. Chicken meats from
small-scale slaughterhouses in Lima, Peru are potential reservoirs of Campylobacter
jejuni and Campylobacter coli and this contamination was associated
to some deficiencies in slaughter process, principally during the evisceration
process. The method presented in this paper has shown to be suitable determination
of Campylobacter species in faecal, meat and water samples.
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How to cite this article:
Cesar A. Lazaro, Carlos A. Conte-Junior, Miguel A. Vilca, Juan R. Lucas, Daphne D. Ramos, Alberto Manchego, Kim R.L. Chiok and Robson M. Franco, 2012. Molecular Identification of Campylobacter jejuni and Campylobacter
coli Isolated from Small-Scale Poultry Slaughterhouse in Lima, Peru. International Journal of Poultry Science, 11: 677-682.
DOI: 10.3923/ijps.2012.677.682
URL: https://scialert.net/abstract/?doi=ijps.2012.677.682
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