Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Poultry Science
  Year: 2010 | Volume: 9 | Issue: 8 | Page No.: 756-760
DOI: 10.3923/ijps.2010.756.760
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Differential Expression of IL-6 and IGF-II in Guinea Fowl and Chicken
G. Goyal, V. Upmanyu, S.K. Singh, S.K. Shukla, S. Mehra, Vinay Kumar and Deepak Sharma

Abstract:
Differential expression of IL-6 and IGF-II genes were studied in guinea fowl and broiler chicken using semi-quantitative analysis. A 219 bp fragment of IL-6 and 215 bp fragment of IGF-II were amplified in guinea fowl and broiler chicken using chicken specific primers. Semi-quantitative analysis revealed the adjusted Integral Density of 0.853 and 0.051 for IL-6 band in guinea fowl and broiler chicken respectively, revealing 16.62 fold higher IL-6 mRNA expression in LPS induced PBMCs from guinea fowl as compared to that from broiler. However, adjusted Integral Density of IGF-II band was 0.082 and 1.106 for IGF-II band in guinea fowl and broiler chicken respectively, which revealed 13.43 fold increase in IGF-II mRNA expression in LPS induced PBMCs in broiler chicken as compared to that in guinea fowl. Hence, guinea fowl showed higher expression of pro-inflammatory cytokine (IL-6) and lower expression of IGF-II in comparison to broiler chicken. These findings were as per expectation in view of much higher immuno-competence and lower growth rate in guinea fowl in comparison to chicken.
PDF References Citation Report Citation
 RELATED ARTICLES:
  •    Simulation Analysis of the Selection of Differential Expression Genes Correlated with Phenotype Data in Chicken Microarray Experiments
  •    Detecting Splicing Variants of FOXP2 and its Protein Expression in Chicken Brain
  •    Glucose Tolerance and Lipid Absorption in Guinea Fowl (Numida meleagris) and Domestic Fowl (Gallus gallus var. domesticus)
How to cite this article:

G. Goyal, V. Upmanyu, S.K. Singh, S.K. Shukla, S. Mehra, Vinay Kumar and Deepak Sharma, 2010. Differential Expression of IL-6 and IGF-II in Guinea Fowl and Chicken. International Journal of Poultry Science, 9: 756-760.

DOI: 10.3923/ijps.2010.756.760

URL: https://scialert.net/abstract/?doi=ijps.2010.756.760

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 

       

       

Curve Bottom