Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Poultry Science
  Year: 2007 | Volume: 6 | Issue: 9 | Page No.: 675-678
DOI: 10.3923/ijps.2007.675.678
The Investigation of Genetic Variation at Microsatellite Loci in Mazandaran Native Chickens
M.T. Beigi Nassiri, Z. Hamidi and S. Tavakoli

Abstract:
Blood samples of Mazandaran native chickens were collected. A total of 90 genomic DNAs were isolated through optimized and modified salting-out procedure. The samples were used in Polymerase Chain Reaction (PCR) with 20 micro satellite markers. Amplified PCR-products with the markers were separated on 8% denaturing polyacrylamide gel. One locus (MCW216) was monomorphic. According to allele frequencies of 20 micro satellite sites, mean heterozygosity (H) and Polymorphism Information Content (PIC) were calculated. The number of alleles varying from 1 to 6 and an estimate of average heterozygosity excluding the monomorphic data was calculated as 0.5872. The average heterozygosity and PIC value calculated from data on polymorphic and monomorphic loci was 0.5579 and 0.4939, respectively. The results of the heterozygosity were consistent with that of PIC. Diversity estimates in this study are lower than the observed frequencies of heterozygotes reported in other species using micro satellite markers. Other parameters for intrapopulation variation and Hardy-Weinberg proportions were also considered. All the loci except MCW222 and MCW165 showed deviations from Hardy-Weinberg equilibrium (p<0.005). Some of micro satellite sites were highly polymorphic, so they were effective markers for genetic diversity analysis. These results could provide basic molecular data for the research on the germplasm characteristics of Mazandaran native chickens.
 [Fulltext PDF]   [View Citation]  [Report Citation]
 RELATED ARTICLES:
  •    Evaluation of Genetic Diversity of Naked Neck and Frizzle Genotypes Based on Microsatellite Markers
  •    Genetic Variation of Three Populations of Indian Frog (Hoplobatrachus tigerinus) Revealed by Allozyme Marker
  •    Molecular Characterization of Different Cytoplasmic Male Sterile Lines Using Mitochondrial DNA Specific Markers in Rice
  •    Genetic Diversity of Wild and Cultured Populations of Penaeus monodon using Microsatellite Markers
How to cite this article:

M.T. Beigi Nassiri, Z. Hamidi and S. Tavakoli, 2007. The Investigation of Genetic Variation at Microsatellite Loci in Mazandaran Native Chickens. International Journal of Poultry Science, 6: 675-678.

DOI: 10.3923/ijps.2007.675.678

URL: https://scialert.net/abstract/?doi=ijps.2007.675.678

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 

Curve Bottom