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International Journal of Plant Pathology

Year: 2012 | Volume: 3 | Issue: 2 | Page No.: 82-88
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Authors

M. Saraswathi

M.N. Reddy

Keywords

Research Article

Phenolic Acids Associated with Sclerotium rolfsii in Groundnut (Arachis hypogaea L.) During Pathogenesis

M. Saraswathi and M.N. Reddy
Stem rot caused by Sclerotium rolfsii is a potential threat to groundnut yield. This disease causes severe damage during any stage of crop growth and yield losses over 25%. Isolation trails from diseased plants were carried out in some areas of Srikalahasti and Tirupati of Chittoor district. Oatmeal-sand culture of S. rolfsii was inoculated to 10 day old seedlings of TMV-2 variety of groundnut for disease development and divided into five fairly distinct stages, on the basis of lesion development. Phenolic extracts were prepared from the hypocotyl regions with lesions and healthy seedlings respectively. The extracts were subjected to 2-dimensional ascending paper chromatographic technique by employing the benzene: acetic acid: water as first solvent system and sodium formate:formic acid:water as the second solvent system. There is a tremendous increase in qualitative and quantitative production of phenolic acids in the diseased area of the groundnut hypocotyls compared to healthy ones. Chromatographic data revealed that there are 10 phenolic acids in healthy plants, whereas in the infected plants, in addition to the above 10 phenolic acids, 6 additional compounds were detected with the progression of the disease.
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