Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Plant Breeding and Genetics
  Year: 2019 | Volume: 13 | Issue: 1 | Page No.: 1-11
DOI: 10.3923/ijpbg.2019.1.11
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Protein-based Fingerprinting in Cowpea Accessions [Vigna uniguiculata (L.) Walp] for Deciphering Genetic Relatedness
J.O. Phillip , O.U. Udensi , I.O. Phillip and S.E. Abimiku

Abstract:
Background and Objective: A clear understanding of the extent of genetic diversity in a germplasm is very critical for maximal exploitation and utilization of inherent resource for conservation and subsequent improvement. This paper was aimed at evaluating the diversity in cowpea accessions using seed storage protein profiling. Materials and Methods: The analysis of the seed storage proteins of cowpea was undertaken at the analytical bioscience laboratory of the International Institute of Tropical Agriculture (IITA) using 15 cowpea accessions obtained from the germplasm of International Institute of Tropical Agriculture (IITA), Ibadan in March, 2017. Seed storage protein was extracted and subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Distinct protein bands were scored for presence (“1”) and absence (“0”) and subjected to analyses using GenAlEx 6.5 and MSVP 3.21 software for genetic parameters. Results: SDS-PAGE analysis confirmed diversity in the seed storage proteins of cowpeas with two major clusters and sub-clusters based on population genetic distance differed according to location and this can be exploited to undertake hybridization of accessions TVu-4467 and/or TVu-8063 from Nigeria as well as TVu-12513 adopted from Zambia. Other genetic diversity parameters indicated the existence of genetic diversity in the 15 accessions. Conclusion: Taking the results together, the investigation confirmed it might be justifiable to carry out parental selection of cowpea accessions adopted from Nigeria, preferably TVu4467 and/or TVu8063 as well as TVu12513 adopted from Zambia for possible crossing to widen the genetic base of cowpea.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Assessing the Genetic Diversity in Cowpea (Vigna unguiculata L. Walp) Accessions Obtained from IITA, Nigeria Using Random Amplified Polymorphic DNA (RAPD)
How to cite this article:

J.O. Phillip, O.U. Udensi, I.O. Phillip and S.E. Abimiku, 2019. Protein-based Fingerprinting in Cowpea Accessions [Vigna uniguiculata (L.) Walp] for Deciphering Genetic Relatedness. International Journal of Plant Breeding and Genetics, 13: 1-11.

DOI: 10.3923/ijpbg.2019.1.11

URL: https://scialert.net/abstract/?doi=ijpbg.2019.1.11

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 

       

       

Curve Bottom