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International Journal of Plant Breeding and Genetics

Year: 2017 | Volume: 11 | Issue: 2 | Page No.: 55-62
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Authors

Durre Shahwar

Durre Shahwar

Mohammad Yunus Khalil Ansari

Towseef Mohsin Bhat

Sana Choudhary

Rumana Aslam

Keywords

Background and Objectives: Genetic variation is indispensable to any plant improvement programme, therefore; this study was primarily based on this aspect of inducing desirable genetic variation for enhancement of the available lentil genetic diversity. The objective of study was to find out the response of caffeine on lentil with a view to distinguished the effect of chemical mutagen on quantitative and qualitative traits in M2 generation. Materials and Methods: In the present experiment, potent chemical mutagen caffeine was used to raise a viable and beneficial mutant at M2 generation level. The seeds (genotype) of lentil were treated with 0.1, 0.25 and 0.50% dose of caffeine prepared in phosphate buffer. Results: Different qualitative and quantitative traits of mutant plant were screened and considerable variations in phenotypic characters were observed. Morphology of plant which is high yielding than its wild type is altered by wide range of macro mutations are called high yielding mutant at 0.25% dose of caffeine. Cytological studies were also done in control as well as mutant plant. Different cytological anomalies such as disturb metaphase with two stray chromosome and stickiness were found. Laggards disturb metaphase II non synchronization and disturbed polarity was also observed in high yielding mutant plant. Conclusion: The optimal dose of caffeine showed accelerating rate of desirable high-yielding mutant had proved to be economical. The segregate of the selected mutant in future generations will definitely contribute to the improvement of lentil genotype and this mutant may be used as valuable breeding stocks for lentil breeding. So by using chemical mutagenesis breeders develop induced mutants in different plants.
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