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International Journal of Plant Breeding and Genetics
  Year: 2014 | Volume: 8 | Issue: 3 | Page No.: 121-138
DOI: 10.3923/ijpbg.2014.121.138
 
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Regeneration of Three Sweet Potato (Ipomoea batatas (L.)) Accessions in Ghana via, Meristem and Nodal Culture
F. Addae-Frimpomaah, J. Amponsah and T.K. Tengey

Abstract:
In vitro regeneration of three sweet potato accessions UE007, UK-BNARI and SA-BNARI using meristem or nodal cuttings was studied. Meristematic explants cultured on (Murashige and Skoog, 1962) basal medium supplemented with low concentration of benzylaminopurine (BAP) or kinetin resulted in callus with or without shoot development which delayed shoot emergence. The degree of callus development increased as the concentration of the cytokinin in the culture medium increased. Although, callus development was comparatively lower on kinetin amended medium than BAP amended medium (Murashige and Skoog, 1962) medium supplemented with 0.25 mg L-1 BAP had the highest shoot induction (80%). For further differentiation of callus or shoots into distinct stem and leaves, the cultures were transferred into fresh MS medium supplemented with 0.25 mg L-1 BAP, 0.1 mg L-1 NAA and 0.1 mg L-1 gibberellic acid. To overcome the delay in shoot initiation using meristem culture, nodal cuttings of sweet potato were used as explants and cultured on MS medium amended with 0.3-0.9 mg L-1 BAP. All explants cultured on 0.3 or 0.6 mg L-1 BAP developed shoots. Post flask acclimatisation of meristem or nodal cutting derived plantlets showed that meristem derived plantlets were better acclimatised than nodal cutting plants due to vigorous root development leading to higher percentage survival in pots and subsequent tuber production as well as other yield parameters. The successful regeneration of sweet potato plantlets in vitro using meristem and nodal cutting explants could be used to complement conventional propagation methods and integration into plant breeding programmes for sweet potato.
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How to cite this article:

F. Addae-Frimpomaah, J. Amponsah and T.K. Tengey, 2014. Regeneration of Three Sweet Potato (Ipomoea batatas (L.)) Accessions in Ghana via, Meristem and Nodal Culture. International Journal of Plant Breeding and Genetics, 8: 121-138.

DOI: 10.3923/ijpbg.2014.121.138

URL: https://scialert.net/abstract/?doi=ijpbg.2014.121.138

 
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