Science Alert
 
Blue
   
Curve Top
International Journal of Pharmacology
  Year: 2022 | Volume: 18 | Issue: 8 | Page No.: 1613-1622
DOI: 10.3923/ijp.2022.1613.1622
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

lncRNA PVT1 Targeting miR-423-5p Regulates Biological Behavior of Gastric Carcinoma Cells

Dazhong Cao, Xilong Ou, Weidong Li and Uzair Ur-Rehman

Abstract:
Background and Objective: Gastric Carcinoma (GC) is categorized among malignant tumours with an extremely increased fatality rate, with an incidence constantly rising in recent years. Accumulating studies have pointed out that lncRNA may be the breakthrough in the fight against tumour diseases. The purpose of this investigation was to explore the mechanism of lncRNA PVT1 on gastric carcinoma cells. Materials and Methods: SGC7901 and MGC803 GC cell multiplication, invasion and apoptosis were studied under altering expressions of PVT1 and miR-423-5p. The interaction between both was further confirmed by the Dual-Luciferase Reporter Assay (DLRA). Results: Silencing PVT1 and increasing miR-423-5p hampered GC cell multiplication and invasiveness capacity and enhanced apoptosis while overexpression had opposite effects (p<0.05). The online target gene prediction website identified a reciprocal binding locus between PVT1 and miR-423-5p and DLRA confirmed the targeting relationship (p<0.05). After inhibiting PVT1, miR-423-5p in GC cells increased (p<0.05). Moreover, the salvage experiment demonstrated that reduced expression levels of PVT1 on GC cells were restored by simultaneously inhibiting miR-423-5p (p>0.05). Conclusion: PVT1 participates in gastric carcinogenesis and progression through the targeted inhibition of miR-423-5p to promote cell activity and reduce apoptosis, which may be a breakthrough in GC research regarding its diagnosis and treatment.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Dazhong Cao, Xilong Ou, Weidong Li and Uzair Ur-Rehman, 2022. lncRNA PVT1 Targeting miR-423-5p Regulates Biological Behavior of Gastric Carcinoma Cells. International Journal of Pharmacology, 18: 1613-1622.

DOI: 10.3923/ijp.2022.1613.1622

URL: https://scialert.net/abstract/?doi=ijp.2022.1613.1622

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 



Curve Bottom