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International Journal of Pharmacology

Year: 2021 | Volume: 17 | Issue: 3 | Page No.: 103-112
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Authors

Dongwei Li

Dongwei Li

Meixia Cao

Yongmin Li

Changqing Qu

Keywords

Research Article

Regulatory Effects of Berberine on Adipogenic Differentiation in Porcine Intramuscular Preadipocytes Through the Multi-Gene Pattern

Dongwei Li Dongwei Li's LiveDNA, Meixia Cao, Yongmin Li and Changqing Qu
Background and Objective: Berberine is well known as a traditional anti-bacterial and anti-inflammatory Chinese medicine. Recently, it was reported to have interesting effects on adipogenic differentiation, glucose uptake, insulin resistance, etc. However, the molecular mechanisms of these underlying pharmacological effects are not sufficiently elucidated. Here, the mechanism of berberine on adipogenic differentiation in porcine intramuscular preadipocytes was investigated. Materials and Methods: Preadipocytes were obtained for primary culture from the healthy piglets under sterile conditions. Preadipocytes viability was detected by MTT assay. Intracellular lipid droplets were observed under microscopy by oil-red O staining and the extraction quantified by colorimetry at room temperature. The key adipogenic genes were detected by real-time quantitative PCR. Luciferase assays were used to validate the targeting relationship between microRNA and the MAPK signalling pathway. Results: When porcine preadipocytes were induced into adipogenic differentiation, cell proliferation and accumulation of intracellular lipid droplets were decreased in a time- and dose-dependent manner by berberine. Berberine attenuated adipogenic differentiation by impairing some key adipogenic genes expression, such as PPARγ and adiponectin and even provoking comprehensive effects through the interference with the interaction of a microRNA (miR-143) and certain a target gene (MAP2K5). Conclusion: The inhibiting effects of berberine on adipogenic differentiation in porcine intramuscular preadipocytes are through the multi-gene pattern, which provides novel evidence for treatments of berberine to gain healthy animal-based foods against excessive fatty deposits, even human obesity, hyperlipidemia, diabetes, etc.
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