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International Journal of Pharmacology

Year: 2020 | Volume: 16 | Issue: 6 | Page No.: 470-478
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Authors

Xiaoying Duan

Xiaoying Duan

Yuanfang Cheng

Feng Sang

Lina Liu

Zhen Liu

Lin Cui

Haixia Li

Keywords

Research Article

Enhanced Targeting Function and Anti-colon Cancer Efficacy by Wheat Germ Agglutinin-modified Nanoparticles for Matrine Delivery

Xiaoying Duan Xiaoying Duan's LiveDNA, Yuanfang Cheng, Feng Sang, Lina Liu, Zhen Liu, Lin Cui and Haixia Li
Background and Objective: A novel delivery system of matrine (MT) using Wheat Germ Agglutinin (WGA), an ideal bio-adhesive material, had been developed by us. The present work was aimed to evaluate the targeting function, anti-colon cancer efficacy and mechanisms of this novel delivery system on cell level. Materials and Methods: After WGA-modified matrine nanoparticles (WGA-MT-NPs) were prepared with our optimizedmethods, the Encapsulation Efficiency (EE) of MT and modification rate of WGA were measured by HPLC and folin-phenol method, respectively. Subsequently, Transmission Electron Microscopy (TEM) was used to further confirm the modification of WGA and HT-29 human colon cancer cells and NCM460 normal colonic epithelial cells were applied to assess the targeting function of WGA-MT-NPs. Furthermore, in HT-29 cells, the cell growth inhibition was determined by tetrazolium salt reagent (MTT) method and the apoptosis and cell cycle arrest were measured usingflowcytometry assay. Results: The EE and modification rate of WGA were 89.99±5.08% and 87.57±8.61%, respectively. And, TEM revealed that WGA was effectively assembled on the surfaces of nanostructures. Moreover, WGA-MT-NPs possessed the specific affinity towards HT-29 cells, rather than NCM460 cells. In HT-29 cells, the growth inhibition rate was significantly improved by WGA-MT-NPs compared with MT-NPs. Furthermore, the apoptosis of HT-29 cells was significantly higher in WGA-MT-NPs than in MT-NPs. Conclusion: WGA-MT-NPs significantly increased the anti-tumor effect of MT-NPs via stronger affinity towards HT-29 cells and higher promotion of apoptosis. Thus, WGA-MT-NPs may provide a promising new strategy for anti-colon cancer.
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How to cite this article

Xiaoying Duan, Yuanfang Cheng, Feng Sang, Lina Liu, Zhen Liu, Lin Cui and Haixia Li, 2020. Enhanced Targeting Function and Anti-colon Cancer Efficacy by Wheat Germ Agglutinin-modified Nanoparticles for Matrine Delivery. International Journal of Pharmacology, 16: 470-478.

DOI: 10.3923/ijp.2020.470.478

URL: https://scialert.net/abstract/?doi=ijp.2020.470.478

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