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International Journal of Pharmacology
  Year: 2019 | Volume: 15 | Issue: 5 | Page No.: 560-566
DOI: 10.3923/ijp.2019.560.566
 
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Anti-melanogenesis Efficacy of Ginkgolide B is Favored by Attenuating Oxidative Stress and Melanin Synthesis in B16f1 Melanoma Cell Model

Zhongfang Zhao, Shaolin Ma and Xianglin Dong

Abstract:
Background and Objective: Ginkgolide B (GB) is a terpene lactone isolated from a popular ancient Chinese tree Ginkgo biloba leaves which possess numerous biological activity. The current study was intended to examine the anti-melanogenic property of GB in B16F1 melanoma cell model by assessing cell viability, free radical production (ROS), tyrosinase activity and melanin content (synthesis). Materials and Methods: B16F1 murine melanoma cells were cultures in DMEM and pre-treated with GB and later stimulated with H2O2 or α-MSH to mimic UV induced hyperpigmentation (hyper-melanogenesis). Results: B16F1 murine melanoma cells (stimulated with H2O2 or α-MSH) and pretreated with increased concentration of GB (5,10, 20 and 50 μg mL1- 1 h before H2O2 or α-MSH stimulation) showed reduced (p<0.001) melanoma cell count (proliferation), lesser (p<0.001) ROS production, inhibit (p<0.01; p<0.001) tyrosinase activity and thus lowered (p<0.001) the melanin content via down regulating (p<0.001) the proteins expression of tyrosinase-related proteins-1 and 2 (TYRP-1 and 2), microphthalmia-associated transcription factor (MITF). Conclusion: This result clearly endowed that GB from Ginkgo biloba leaves showed better anti-melanogenic property in a dose-dependent fashion by abolishing excessive ROS generation (oxidative stress) as well as effectively inhibited tyrosinase activity and thereby suppress the production of melanin which reflects in decreased melanin count. Hence, it recommended that GB could be used as a skin whitening agent or derma-protective agent in the cosmetic industry.
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How to cite this article:

Zhongfang Zhao, Shaolin Ma and Xianglin Dong, 2019. Anti-melanogenesis Efficacy of Ginkgolide B is Favored by Attenuating Oxidative Stress and Melanin Synthesis in B16f1 Melanoma Cell Model. International Journal of Pharmacology, 15: 560-566.

DOI: 10.3923/ijp.2019.560.566

URL: https://scialert.net/abstract/?doi=ijp.2019.560.566

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