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International Journal of Pharmacology
  Year: 2019 | Volume: 15 | Issue: 5 | Page No.: 533-541
DOI: 10.3923/ijp.2019.533.541
 
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Inhibitory Effect of Grape Seed Polyphenol Extract and Vitamin C on Melanogenesis in Cultured B16-F1 Melanoma Cells

Hui-Fang Chiu, Shine-Ling Huang, You-Cheng Shen, Yi-Chun Han, Kamesh Venkatakrishnan and Chin-Kun Wang

Abstract:
Background and Objective: The grape seed polyphenol (GSP) is rich in flavonoids and tannins which contributes to diverse pharmacological activities. The current study was undertaken to assess the anti-melanogenesis effect of GSPE on UVA-exposed B16F1 melanoma cells by checking morphological and biochemical changes. Materials and Methods: The present study was sub-divided into three phases. In phase I, the anti-melanogenesis effect of crude grape seed polyphenol extract (GSPE) was checked by comparing with three commercial grape products (A, B and C) in non-UVA exposed B16F1 cells. During phase II, the anti-melanogenesis effect of GSPE was determined on UV-exposed B16F1 cells to reveal the effective dosage. Finally, in phase III the anti-melanogenesis effect of different experimental samples (GSPE; 50 μg, Vit C; 1 μg and combo of GSPE- Vit C; 50:1μg) were examined on UV-exposed B16F1 cells. Results: The crude GSPE at the dose of 50 μg exhibited better (p<0.05) melanin inhibitory activity (suppressed melanin synthesis) than commercial grape products (phase I) as well as successfully attenuated (p<0.05) UV-induced photo-oxidative stress by its free radical scavenging activity in phase II. All the experimental samples in phase III presented anti-melanogenesis activity, but crude GSPE (50 μg) displayed the greatest (p<0.05) melanin inhibitory activity by down regulating tyrosinase activity, thus proving its anti-melanogenesis activity. Conclusion: In this study, crude GSPE (50 μg) showed the maximum melanin inhibitory activity as it successfully down regulated the tyrosinase activity.
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How to cite this article:

Hui-Fang Chiu, Shine-Ling Huang, You-Cheng Shen, Yi-Chun Han, Kamesh Venkatakrishnan and Chin-Kun Wang, 2019. Inhibitory Effect of Grape Seed Polyphenol Extract and Vitamin C on Melanogenesis in Cultured B16-F1 Melanoma Cells. International Journal of Pharmacology, 15: 533-541.

DOI: 10.3923/ijp.2019.533.541

URL: https://scialert.net/abstract/?doi=ijp.2019.533.541

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