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International Journal of Pharmacology
  Year: 2019 | Volume: 15 | Issue: 4 | Page No.: 509-514
DOI: 10.3923/ijp.2019.509.514
Effects of Minocycline on H2O2-induced Cell Death and Interleukin-8 Production in Human Small Airway Epithelial Cells
Takahiro Sato, Ayano Hirohara, Yuki Izumi, Takayuki Nakatomi, Kuninori Iwayama, Ko-Ichi Ohtaki and Nobumasa Hayase

Abstract:
Background and Objective: The antibiotic minocycline (MINO) is known to have anti-inflammatory activity. This study aimed to examine the effects of MINO on hydrogen peroxide (H2O2)-induced cell death and interleukin (IL)-8 production, which have been shown to increase during inflammation, using small airway epithelial cells (SAECs). Materials and Methods: The SAECs were pretreated with MINO (1, 5 and 10 μM) for 24, 48 or 72 h and subsequently exposed to H2O2 (100 μM) for 6 h. Cell viability was assessed using the WST-8 assay and levels of IL-8 protein were measured using an enzyme-linked immunosorbent assay (ELISA). Tukey’s multiple comparison test was used for analysis of statistical significance. Results: Treatment of SAECs with 10 μM MINO or greater than 100 μM H2O2 significantly decreased cell viability. However, pretreatment with 1 μM MINO for 24 h significantly suppressed the H2O2-induced decrease in cell viability. In addition, IL-8 production increased in the H2O2 treated group as compared to the control group (not treated with H2O2), whereas IL-8 production decreased significantly following pretreatment with MINO (1, 5 or 10 μM) for 48 or 72 h. Conclusion: It was suggested that pretreatment of SAECs with low concentrations of MINO for 24 or 48 h has a cytoprotective effect and pretreatment for 48 or 72 h suppresses IL-8 production indicating a potential anti-inflammatory effect.
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How to cite this article:

Takahiro Sato, Ayano Hirohara, Yuki Izumi, Takayuki Nakatomi, Kuninori Iwayama, Ko-Ichi Ohtaki and Nobumasa Hayase, 2019. Effects of Minocycline on H2O2-induced Cell Death and Interleukin-8 Production in Human Small Airway Epithelial Cells. International Journal of Pharmacology, 15: 509-514.

DOI: 10.3923/ijp.2019.509.514

URL: https://scialert.net/abstract/?doi=ijp.2019.509.514

 
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